Role of complement 3 in TNF-α-induced mesenchymal transition of renal tubular epithelial cells in vitro.

Molecular Biotechnology
Jian-xin WanDanyu You

Abstract

Injured renal tubular epithelial cells (RTECs) have been recently thought to directly contribute to the accumulation of myofibroblasts in renal tubulointerstitial fibrosis through a process of epithelial to mesenchymal transition (EMT). However, the factors inducing RTECs to undergo EMT and the underlying mechanisms need to be further elucidated. This study aimed to determine the EMT-inducing activity of proinflammatory cytokine TNF-α and the role for complement 3 (C3) in this activity in an in vitro model of human RTECs (HK-2 cells). Wild type HK-2 cells were treated with TNF-α, IFN-γ or C3a; C3 siRNA- or control siRNA-carrying HK-2 cells were treated with TNF-α. Changes in the cell morphology and phenotype were assessed by microscopy, RT-PCR, western blotting, and immunostaining. TNF-α effectively induced HK-2 cells to express C3 and to transform into morphologically myofibroblast-like cells that lost E-cadherin (a classical epithelial cell marker) expression but acquired alpha-smooth muscle actin (α-SMA, a classical myofibroblast differentiation marker) expression. C3 siRNA robustly attenuated all the morphologic and phenotypic changes induced by TNF-α but the control siRNA showed no effect. Our preliminary observations sugg...Continue Reading

References

Jan 1, 1997·Clinical and Experimental Immunology·B P Morgan, P Gasque
Nov 13, 1998·The New England Journal of Medicine·G Remuzzi, T Bertani
May 17, 2000·The Journal of Clinical Investigation·W ZhouS H Sacks
Sep 29, 2000·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·M R Daha, C van Kooten
Jun 4, 2002·Nature Medicine·Julian R PrattSteven H Sacks
Dec 18, 2003·The Journal of Clinical Investigation·Raghu Kalluri, Eric G Neilson
Feb 2, 2006·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Conrad A FarrarSteven H Sacks
Sep 5, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Daniel TurnbergH Terence Cook
Oct 13, 2006·Journal of the American Society of Nephrology : JASN·Frank Strutz, Michael Zeisberg
May 24, 2007·Journal of Cellular Physiology·Jian-Xin WanKoichi Matsumoto
Nov 28, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Neil S SheerinSteven H Sacks
Dec 6, 2008·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Paul L DudasFrancis X Farrell
Jan 23, 2009·Journal of the American Society of Nephrology : JASN·Ziyong TangNeil S Sheerin

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Citations

Dec 7, 2013·Apoptosis : an International Journal on Programmed Cell Death·Tian-Biao ZhouGregor P C Drummen
Jun 23, 2015·Journal of Receptor and Signal Transduction Research·Tian-Biao ZhouFeng Zhang
Sep 28, 2015·Biochemical and Biophysical Research Communications·Takayuki AdachiTomohiro Kato
Mar 31, 2016·Journal of Clinical Medicine·Anai N KothariPaul C Kuo
Jan 31, 2018·Scientific Reports·Martin J PageEtheresia Pretorius
Dec 20, 2019·Hypertension Research : Official Journal of the Japanese Society of Hypertension·Lan ChenMasanori Abe
Jan 1, 2016·The Journal of Immunology : Official Journal of the American Association of Immunologists·Min Soon ChoVahid Afshar-Kharghan
Mar 2, 2017·The Journal of Clinical Investigation·Vahid Afshar-Kharghan

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