Role of complement in rats injected with liposome-encapsulated hemoglobin

The Journal of Surgical Research
B GoinsA S Rudolph

Abstract

Previous studies have documented that liposome-encapsulated hemoglobin (LEH) can cause a rapid and transient thrombocytopenia following intravenous injection into small animals. The present study evaluated the role of complement during the LEH-induced thrombocytopenia in rats. We have compared changes in platelet levels in the blood, platelet organ distribution, and total hemolytic complement levels following intravenous administration of LEH in control and complement-depleted rats. Changes in platelet organ distribution at various times after LEH administration were monitored by labeling autologous platelets with indium-111 (111In)-oxine and imaging the 111In-platelets with a gamma camera after reinjection. Platelet counts were determined by light-scattering methods and by following 111In radioactivity at various times after LEH administration. Platelet levels did not significantly change for the complement-depleted rats during the 60 min following an injection of LEH, whereas thrombocytopenia (40% decrease) was noted within 4 min post-LEH-injection for control rats with a gradual return to baseline circulating platelet levels within 60 min. This drop in circulating platelets was correlated with a rapid redistribution of 111In...Continue Reading

References

Aug 1, 1992·The Journal of Surgical Research·C CardozoM Lesser
Jan 1, 1985·International Anesthesiology Clinics·G M Vercellotti, D E Hammerschmidt
Mar 19, 1971·Brain Research·J C Liebeskind, D J Mayer
Mar 21, 1983·FEBS Letters·B P GaberE L Chang
Feb 1, 1980·British Journal of Haematology·A D HeynsF P Retief
Jan 1, 1995·Artificial Cells, Blood Substitutes, and Immobilization Biotechnology·J SzebeniC R Alving
Apr 20, 1994·Biochimica Et Biophysica Acta·D V DevineP R Cullis
Sep 1, 1993·Chemistry and Physics of Lipids·N M Wassef, C R Alving
Dec 1, 1958·Proceedings of the Society for Experimental Biology and Medicine·A L LEVY
Oct 1, 1992·Clinical Nutrition : Official Journal of the European Society of Parenteral and Enteral Nutrition·P E DahlJ C Kjæve

❮ Previous
Next ❯

Citations

May 23, 2012·Journal of Biomedical Materials Research. Part a·Hiromi SakaiMayumi Kano
May 4, 2002·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Guohui RenRichard J Quigg
Aug 31, 2000·Critical Care Medicine·J CreteurJ L Vincent
Jan 16, 2004·Critical Care Medicine·Jacques Creteur, Jean-Louis Vincent
Mar 4, 1999·Artificial Cells, Blood Substitutes, and Immobilization Biotechnology·J Szebeni, C R Alving
Dec 16, 2003·Artificial Cells, Blood Substitutes, and Immobilization Biotechnology·Iren ConstantinescuMaria Gyongyossy-Issa
Sep 12, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·László DézsiGábor Szénási
May 19, 2001·Artificial Cells, Blood Substitutes, and Immobilization Biotechnology·S WakamotoK Ikebuchi
Apr 30, 2019·Frontiers in Bioengineering and Biotechnology·Suyun Janet TanShinji Takeoka
Nov 10, 2009·Colloids and Surfaces. B, Biointerfaces·Hrushikesh AgasheVibhudutta Awasthi

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2021 Meta ULC. All rights reserved