Role of constitutively expressed heme oxygenase-2 in the regulation of guinea pig coronary artery tone

Pflügers Archiv : European journal of physiology
Hristo GagovDessislava Duridanova

Abstract

Carbon monoxide (CO) is well known as a relaxing substance in the vasculature, where it is released during the heme oxygenase (HO) reaction. Little is known about the tissue-specific targets of CO in smooth muscles. To date the functional role of CO in the coronary artery remains unclear. The expression of HO-2, the constitutive isoform of HO, but not of HO-1 (inducible HO isoform) was demonstrated by immunohistochemical reaction. Contractile studies, performed under isometrical conditions, showed that CO, as well as hemin (given as a substrate for HO), relax de-endothelized coronary smooth muscle after the blockade of neuronal transmission. The action of hemin was antagonized by preliminary treatment of the vessel with SnPPIX--a competitive inhibitor of HO. The relaxatory effects of hemin were abolished in the presence of guanylyl-cyclase or protein kinase G antagonists. Patch-clamp studies revealed that hemin caused activation of iberiotoxin-blockable K outward current (I(K)) via guanylyl-cyclase and protein-kinase-G-dependent mechanisms. This activation coincided with hyperpolarization of the plasma membrane of single coronary smooth muscle cells by 8+/-3 mV, which was prevented by preliminary exposure of cells to 10 microM ...Continue Reading

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Citations

Sep 2, 2004·Pflügers Archiv : European journal of physiology·Kiril HristovDessislava Duridanova
Mar 6, 2010·Hypertension Research : Official Journal of the Japanese Society of Hypertension·Joseph Fomusi Ndisang, Ashok Jadhav
Oct 23, 2009·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·David E StecMichael J Ryan
Jan 4, 2012·BioFactors·Francesca GullottaPaolo Ascenzi
Dec 14, 2006·Prostaglandins & Other Lipid Mediators·David SacerdotiNader G Abraham
May 29, 2004·Journal of Hypertension·Joseph F NdisangRui Wang
May 19, 2004·American Journal of Physiology. Heart and Circulatory Physiology·Yasuhiro NishikawaWilliam M Chilian
May 14, 2008·Journal of Hypertension·Joseph Fomusi NdisangAshok Jadhav
Jun 27, 2006·American Journal of Physiology. Heart and Circulatory Physiology·David SacerdotiNader G Abraham
Aug 21, 2007·Essays in Biochemistry·Michael L Paffett, Benjimen R Walker
Oct 28, 2019·Bulletin of Experimental Biology and Medicine·A E KotsyubaI A Khramova

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