Role of COX-2 in microcirculatory disturbance in experimental pancreatitis

World Journal of Gastroenterology : WJG
Wen-Wei YanHong-Kai Gao

Abstract

To elucidate the role of COX-2 in the development of capillary leakage in rats with acute interstitial pancreatitis. Rats with acute interstitial pancreatitis were induced by caerulein subcutaneous injection. Reverse transcription-polymerase chain reaction (RT-PCR) was used to determine the gene expression of COX-2 in pancreatic tissues, spectrophotometry was used to assay the parameters of acute pancreatitis such as the serum amylase and plasma myeloperoxidase, and determination of capillary permeability in the pancreas by quantifying the permeability index (PI) assisted response of pancreatic microvascular via intravital fluorescence microscope video image analysis system. A significant increase of COX-2 expression, elevation of serum amylase, and plasma myeloperoxidase were detected in rats with acute edematous pancreatitis compared with control rats. The changes of pancreatic microvascular after caerulein injection were as following: (a) the decrease of pancreatic capillary blood flow (4th h, 0.56+/-0.09 nL/min, P<0.05; 8 th h, 0.34+/-0.10 nL/min, P<0.001); (b) reduction of functional capillary density (4 th h, 381+/-9 cm(-1), P>0.05; 8th h, 277+/-13 cm(-1), P<0.001); (c) irregular and intermittent capillary perfusion was o...Continue Reading

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Citations

Jul 4, 2006·ANZ Journal of Surgery·Christine M Cuthbertson, Christopher Christophi
Jan 2, 2007·Pancreas·Yuk Cheung Chan, Po Sing Leung
Dec 16, 2006·World Journal of Gastroenterology : WJG·Jian-Xin ZhangXue-Qing Wang
Jan 26, 2017·BioMed Research International·Guotao LuWeiqin Li
May 24, 2006·World Journal of Gastroenterology : WJG·Qiang YanChun-Fan Han
Apr 12, 2006·The British Journal of Surgery·C M Cuthbertson, C Christophi
Jan 1, 2008·PPAR Research·Raghu Vemuganti
Jan 15, 2008·Pancreas·Christine M CuthbertsonChristopher Christophi
Mar 7, 2020·The American Journal of Gastroenterology·Zhiyin HuangLibin Huang

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