Role of CPI-17 in the regulation of endothelial cytoskeleton

American Journal of Physiology. Lung Cellular and Molecular Physiology
Irina KolosovaAlexander D Verin

Abstract

We have previously shown that myosin light chain (MLC) phosphatase (MLCP) is critically involved in the regulation of agonist-mediated endothelial permeability and cytoskeletal organization (Verin AD, Patterson CE, Day MA, and Garcia JG. Am J Physiol Lung Cell Mol Physiol 269: L99-L108, 1995). The molecular mechanisms of endothelial MLCP regulation, however, are not completely understood. In this study we found that, similar to smooth muscle, lung microvascular endothelial cells expressed specific endogenous inhibitor of MLCP, CPI-17. To elucidate the role of CPI-17 in the regulation of endothelial cytoskeleton, full-length CPI-17 plasmid was transiently transfected into pulmonary artery endothelial cells, where the background of endogenous protein is low. CPI-17 had no effect on cytoskeleton under nonstimulating conditions. However, stimulation of transfected cells with direct PKC activator PMA caused a dramatic increase in F-actin stress fibers, focal adhesions, and MLC phosphorylation compared with untransfected cells. Inflammatory agonist histamine and, to a much lesser extent, thrombin were capable of activating CPI-17. Histamine caused stronger CPI-17 phosphorylation than thrombin. Inhibitory analysis revealed that PKC mo...Continue Reading

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Citations

Jun 2, 2011·Cold Spring Harbor Perspectives in Biology·Peter Lipp, Gregor Reither
Jul 1, 2008·American Journal of Physiology. Lung Cellular and Molecular Physiology·Csilla CsortosAlexander D Verin
Mar 8, 2011·American Journal of Physiology. Lung Cellular and Molecular Physiology·Paula J BernalClaudette M St Croix
Jun 23, 2012·PloS One·Takako Kaneko-KawanoKenji Suzuki
Apr 16, 2013·Microvascular Research·Kyung-mi KimAlexander D Verin
Feb 4, 2012·Experimental Eye Research·Ariadna Yolanda Ruiz-LoredoAna María López-Colomé
Feb 24, 2009·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Bernadett KolozsváriEva Bakó
Aug 23, 2006·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Akio MaedaShinya Kuroda
Jun 17, 2011·Journal of Cellular Physiology·Kyung-Mi KimAlexander D Verin
Nov 16, 2018·Journal of Obstetrics and Gynaecology : the Journal of the Institute of Obstetrics and Gynaecology·Qiuping LiaoRongxin Chen
Feb 14, 2006·Journal of Cellular Biochemistry·Krisztina TarAlexander D Verin
Nov 9, 2014·American Journal of Physiology. Lung Cellular and Molecular Physiology·Dolly MehtaWolfgang M Kuebler
Feb 22, 2011·American Journal of Physiology. Lung Cellular and Molecular Physiology·Sarah L Sayner
Oct 12, 2007·Journal of Cell Science·Stéphanie Pellegrin, Harry Mellor

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