Abstract
Use of muscle grafts for hemostasis during surgery has re-emerged; recent animal model studies have shown effective bleeding control with their use. However, the mechanism of action is unknown. The aim of this study is to evaluate the action of muscle extracts on the coagulation pathways and platelet aggregation. Muscle extracts were prepared by dissolving crushed snap-frozen muscle tissue (0.04 to 0.8 mg) in 1 mL saline. Saline was used as control. Prothrombin time, activated partial thromboplastin time (APTT), thrombin time, and platelet aggregation studies were performed on both muscle extract and saline. Prothrombin time and APTT were repeated using factor VII-deficient plasma, factor X-deficient plasma, lupus plasma, and contact pathway-inhibited plasma. Mean readings in the muscle group and control group were compared using nonparametric Mann-Whitney U test (Wilcoxon rank sum test with continuity correction). Among the various coagulation parameters, there was no significant difference between saline and muscle (p > 0.05), except in the APTT using factor X-deficient plasma (mean APTT 133.89 seconds and 185.10 seconds for muscle and saline, respectively; p < 0.0001). Higher concentrations of the muscle extract (>0.5 mg/mL)...Continue Reading
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