PMID: 7544116Aug 15, 1995Paper

Role of cyclic nucleotides in store-mediated external Ca2+ entry in human platelets

The Biochemical Journal
K NakamuraA Aviv

Abstract

This study explores the role of cyclic nucleotides (i.e. cyclic AMP and cyclic GMP) in store-regulated external Ca2+ entry in human platelets. To stimulate store-regulated external Ca2+ entry, thapsigargin was used to deplete Ca2+ from the dense tubules, and sodium nitroprusside and iloprost respectively were used to stimulate endogenous cyclic GMP and cyclic AMP formation. Pretreatment with sodium nitroprusside and iloprost (a) attenuated the thapsigargin-evoked external Ca2+ entry and (b) reduced the rate of Ca2+ release from the dense tubules. The effects on external Ca2+ entry and Ca2+ release from the dense tubules were exerted independently and were apparently mediated through activation of the respective cyclic nucleotide-dependent protein kinases. Both sodium nitroprusside and iloprost reduced tyrosine kinase phosphorylation of a number of proteins, particularly a 72 kDa protein band. Both agents also attenuated the thapsigargin-evoked tyrosine kinase phosphorylation of the 72 kDa band. Intracellular Ca2+ depletion resulted in a reduction in tyrosine kinase-mediated phosphorylation of a number of protein bands, including the 72 kDa band and the further attenuation of thapsigargin-mediated tyrosine phosphorylation of thi...Continue Reading

Citations

Mar 10, 2001·The Journal of Clinical Investigation·J E FabreB H Koller
Oct 31, 2002·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·Dagnia LoomsSteen Dissing
Dec 3, 2020·International Journal of Molecular Sciences·Steffen BrauneFriedrich Jung
Aug 1, 1997·Trends in Pharmacological Sciences·E Clementi, J Meldolesi

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