PMID: 6165635Apr 1, 1981Paper

Role of cyclic nucleotides in the inhibition of growth-hormone secretion by somatostatin

Molecular and Cellular Endocrinology
R J Bicknell, J G Schofield

Abstract

The effects of somatostatin on GH secretion, cyclic AMP and cyclic GMp concentrations in dispersed bovine anterior pituitary cells were studied following activation of adenylate cyclase with cholera toxin and inhibition of phosphodiesterase with isobutylmethylxanthine (IBMX). Cholera toxin (10(-5)M) increased intracellular cyclic AMP concentration 10-fold and cyclic GMP concentration 3-fold relative to control, and stimulated the secretion of GH. IBMX (10(-4) M) als increased intracellular concentrations of both cyclic AMP and cyclic GMP and the secretion of GH and potentiated the actions of cholera toxin particularly in raising intracellular cyclic AMP concentrations which were elevated 40-fold in the presence of cholera toxin and IBMX. Somatostatin (5 X 10(-7) M) completely prevented GH secretion elicited by cholera toxin and/or IBMX. Somatostatin was without effect on control cyclic AMP and cyclic GMp concentrations and on the increases in both cyclic AMP and cyclic GMP caused by cholera toxin and by IBMX alone, or in combination. The data suggest that bovine GH secretion is increased when concentrations of either or both cyclic nucleotides are elevated within the cells, although incubation of cells with extracellular concen...Continue Reading

References

Jan 1, 1977·Annual Review of Biochemistry·N D Goldberg, M K Haddox
Feb 7, 1977·Biochemical and Biophysical Research Communications·T Maruyama, H Ishikawa
Feb 1, 1979·Molecular and Cellular Endocrinology·R J BicknellJ G Schofield
Apr 13, 1979·Biochemical and Biophysical Research Communications·J W LeitnerK E Sussman
Jul 1, 1979·Proceedings of the Society for Experimental Biology and Medicine·N Hayasaki-Kimura, K Takahashi
Jan 1, 1978·Molecular and Cellular Endocrinology·J G Schofield, R J Bicknell
Mar 1, 1974·Nature·R S Rappaport, N H Grant

❮ Previous
Next ❯

Citations

Jul 1, 1982·Molecular and Cellular Endocrinology·K P Ray, M Wallis
Mar 1, 1984·Molecular and Cellular Endocrinology·B Asgeirsson, J G Schofield
Aug 1, 1986·Clinics in Endocrinology and Metabolism·B B Bercu, F B Diamond
Apr 1, 1983·The American Journal of Physiology·M J CroninM O Thorner

❮ Previous
Next ❯

Related Concepts

Related Feeds

Aphasia

Aphasia affects the ability to process language, including formulation and comprehension of language and speech, as well as the ability to read or write. Here is the latest research on aphasia.