Role of cysteinyl leukotrienes in human allergen-specific Th2 responses induced by granulocyte macrophage-colony stimulating factor

Allergy
A FaithC M Hawrylowicz

Abstract

The pro-inflammatory cytokine, granulocyte macrophage-colony stimulating factor (GM-CSF), which is elevated in the lungs of atopic asthmatic patients, has been shown to enhance major histocompatibility class II expression of alveolar macrophages (AM). We hypothesized that exposure of AM and monocytes from atopic asthmatic patients to GM-CSF would enhance their antigen presenting function, and investigated putative mechanisms for this effect. Alveolar macrophages were purified from bronchoalveolar lavage by plastic adherence. Monocytes and CD4(+) T cells were purified from peripheral blood by magnetic bead separation. Antigen-presenting cell (APC) were pretreated with GM-CSF, pulsed with allergen and cocultured with autologous T cells. T-cell proliferation was determined by tritiated thymidine incorporation and cytokine production by enzyme-linked immunosorbent assay. Exposure of allergen-pulsed AM and peripheral blood monocytes to GM-CSF significantly increased allergen-specific T-cell proliferation and T helper 2 (Th2) cytokine production. The enhanced response was dependent on costimulation by CD86, but not CD80. Inhibition of the 5-lipoxygenase pathway abrogated GM-CSF-mediated upregulation by monocytes of allergen-specific ...Continue Reading

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Citations

Jun 23, 2009·Journal of Reproductive Immunology·Martina SandbergMaria C Jenmalm
Jan 25, 2011·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·J P McFaddenI Kimber
Nov 26, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Yiming Zhu, Paul J Bertics
Dec 17, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Luzheng XueRoy Pettipher
Jul 5, 2013·American Journal of Respiratory Cell and Molecular Biology·Xiaoju TangFengming Luo
Jun 17, 2020·The Journal of Allergy and Clinical Immunology·Pascal HaimerlJulia Esser-von Bieren

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