PMID: 8600980Mar 15, 1996Paper

Role of cytochrome P-450 in the stimulation of microsomal production of reactive oxygen species by ferritin

Biochimica Et Biophysica Acta
S Puntarulo, A I Cederbaum

Abstract

Microsomes can remove iron from ferritin by a superoxide-dependent reaction. The released iron can then catalyse formation of a variety of reactive oxygen species. Experiments were carried out to evaluate the role of cytochrome P-450 in the release of iron from ferritin, and whether induction of certain P-450 isoforms alters ferritin-dependent reactive oxygen radical production. Rats were treated with phenobarbital, 3-methylcholanthrene, 4-methylpyrazole, or saline to produce microsomes with varying P-450 content and composition. Oxidation of 2,7'-dichlorofluorescein diacetate to a fluorescent product and chemiluminescence were used as indices of production of reactive oxygen species. The extreme sensitivity of these reactions to trolox, a potent chain-breaking oxidant, indicates the involvement of lipid peroxidation products in these reactions. In the absence of ferritin, formation of reactive oxygen species was higher in microsomes from the treated rats compared to saline controls when results were expressed on a per mg protein basis but not per nmol P-450, suggesting that the increased content of total P-450 (2-fold increases) rather than the population of isoforms was responsible for the increase. Superoxide dismutase had n...Continue Reading

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Citations

Mar 31, 2004·Molecular Aspects of Medicine·Monica GalleanoSusana Puntarulo
Aug 5, 2009·Toxicology·Ivan Rousseau, Susana Puntarulo
Mar 10, 2009·Archives of Physiology and Biochemistry·Kara C SedorisAndrew M Roberts
Aug 17, 2005·Molecular Aspects of Medicine·Susana Puntarulo
Apr 1, 1997·Archives of Biochemistry and Biophysics·S Puntarulo, A I Cederbaum
Jul 16, 2002·Arteriosclerosis, Thrombosis, and Vascular Biology·Haoyi ZhengStuart D Katz
Jun 21, 2006·Trauma, Violence & Abuse·T K LoganTeri Marie Faragher
Apr 12, 2001·European Journal of Biochemistry·S IamettiF Bonomi
Feb 15, 2001·Journal of Biomedical Science·A Y Sun, G Y Sun
Nov 20, 2003·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Laura P JamesJack A Hinson
Dec 22, 1999·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·M Strolin-BenedettiJ G Hengstler
Dec 6, 1996·Biochimica Et Biophysica Acta·A Caro, S Puntarulo

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