Role of DetR in defence is critical for virulence of Xanthomonas oryzae pv. oryzae

Molecular Plant Pathology
Minh-Phuong NguyenSang-Won Lee

Abstract

Like other bacteria, Xanthomonas oryzae pv. oryzae (Xoo), the causal agent of bacterial leaf blight disease in rice, possesses intracellular signalling systems, known as two-component regulatory systems (TCSs), which regulate pathogenesis and biological processes. Completion of the genome sequences of three Xoo strains has facilitated the functional study of genes, including those of TCSs, but the biological functions of most Xoo TCSs have not yet been uncovered. To identify TCSs involved in Xoo pathogenesis, we generated knockout strains lacking response regulators (RRs, a cytoplasmic signalling component of the TCS) and examined the virulence of the RR knockout strains. This study presents a knockout strain (detR(-) ) lacking the PXO_04659 gene which shows dramatically reduced virulence relative to the wild-type. Our studies to elucidate detR function in Xoo pathogenesis revealed a reduction in extracellular polysaccharide (EPS), intolerance to reactive oxygen species (ROS) and deregulation of iron homeostasis in the detR(-) strain. Moreover, gene expression of regulatory factors, including other RRs and transcription factors (TFs), was altered in the absence of DetR protein, as determined by reverse transcription-polymerase ...Continue Reading

References

Dec 1, 1975·Carbohydrate Research·P E JanssonB Lindberg
Jan 1, 1987·Analytical Biochemistry·B Schwyn, J B Neilands
Dec 1, 1995·Toxicology Letters·C C Winterbourn
May 30, 1998·Proceedings of the National Academy of Sciences of the United States of America·J SchultzC P Ponting
Oct 9, 1998·Applied Microbiology and Biotechnology·A BeckerL Ielpi
Jul 13, 2000·Cellular and Molecular Life Sciences : CMLS·J DatF Van Breusegem
May 5, 2001·Trends in Microbiology·I W Sutherland
Jun 19, 2001·Trends in Biochemical Sciences·A H West, A M Stock
Aug 30, 2001·Applied Microbiology and Biotechnology·R Sunish Kumar, N Sakthivel
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
May 31, 2002·Molecular Plant-microbe Interactions : MPMI·Subhadeep Chatterjee, Ramesh V Sonti
Jun 28, 2003·FEMS Microbiology Reviews·Simon C AndrewsFrancisco Rodríguez-Quiñones
Jan 28, 2004·Cellular and Molecular Life Sciences : CMLS·P ChelikaniP C Loewen
Jun 1, 1997·Annual Review of Plant Physiology and Plant Molecular Biology·Chris Lamb, Richard A. Dixon
Jun 16, 2004·Molecular Plant-microbe Interactions : MPMI·Saul BurdmanPamela Ronald
Jan 11, 2005·Trends in Microbiology·Steven S BrandaRoberto Kolter
Jul 27, 2005·Molecular Plant-microbe Interactions : MPMI·Sujatha Subramoni, Ramesh V Sonti
Mar 15, 2006·Plant Physiology·Maximina H YunAdrián A Vojnov
Jan 26, 2007·Molecular Plant-microbe Interactions : MPMI·Gopaljee JhaRamesh V Sonti
Dec 14, 2007·Annual Review of Genetics·Michael T Laub, Mark Goulian
Feb 12, 2008·Current Protocols in Molecular Biology·B Seed
Mar 4, 2008·Microbial Pathogenesis·Hee-Jung ChoByoung-Moo Lee
Jul 22, 2008·Current Biology : CB·Shazia N AslamRichard M Cooper
Nov 11, 2008·Journal of Visualized Experiments : JoVE·Alexandrine Froger, James E Hall
Jan 1, 1995·Annual Review of Phytopathology·T P Denny
Oct 24, 2009·Molecular Plant Pathology·Frank F WhiteRalf Koebnik
Nov 21, 2009·FEMS Microbiology Reviews·Daniela Büttner, Ulla Bonas
Jun 15, 2010·BMC Microbiology·Mauricio Soto-SuárezValérie Verdier

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