Role of different medium and growth factors on placental blood stem cell expansion: an in vitro and in vivo study

Bone Marrow Transplantation
M BergerM Aglietta

Abstract

Expansion of haemopoietic stem cells from placental blood has been obtained with a combination of flt3 ligand (FL), thrombopoietin (TPO), kit-ligand (KL) with or without interleukin-6 (IL6) in serum-replete medium. For clinical use, cell expansion in the absence of serum is a clear advantage. Therefore, stem cell expansion in serum-free (SF) medium with a combination of three (FL, TPO, KL) or four (FL, TPO, KL, IL6) growth factors was compared with the results obtained using fetal calf serum (FCS) or human serum (HS). Human CD34(+) placental blood cells were cultured in the presence of FL, TPO, KL +/- IL6 with SF medium, HS and FCS for up to 8 weeks. CD34(+), CFC, LTC-IC content was measured at intervals. To determine the in vivo repopulating capacity of expanded cells, CD34(+) expanded cells were transplanted in sublethally irradiated NOD/SCID mice. With the three growth factor combination the CD34(+) cell number increased steadily up to the 8 weeks of culture. CD34(+) cells were expanded 67.5-fold with SF, 11.7 with HS and 49.2 with FCS. However, when CFCs and LTC-ICs were considered, a continuous expansion was observed only with HS and FCS, whereas in SF medium after 6 weeks their number started to decline. The addition of I...Continue Reading

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Citations

Jun 6, 2006·Laboratory Hematology : Official Publication of the International Society for Laboratory Hematology·Azza A MohamedRaafat M Riad
May 4, 2011·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Julie LangRoberta Pelanda
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Nov 27, 2014·World Journal of Stem Cells·Francesca BianchiGaetano La Manna
Nov 2, 2013·World Journal of Stem Cells·Maya FakhryDavid Magne
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Aug 6, 2003·Blood·Pieternella S In 't AnkerHumphrey H H Kanhai
Sep 21, 2004·Oncogene·Guy SauvageauR Keith Humphries

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