Role of DNA in the activation of the Cry1A insecticidal crystal protein from Bacillus thuringiensis.

The Journal of Biological Chemistry
F R ClairmontH Kaplan

Abstract

The Cry1A insecticidal crystal protein (protoxin) from six subspecies of Bacillus thuringiensis as well as the Cry1Aa, Cry1Ab, and Cry1Ac proteins cloned in Escherichia coli was found to contain 20-kilobase pair DNA. Only the N-terminal toxic moiety of the protoxin was found to interact with the DNA. Analysis of the crystal gave approximately 3 base pairs of DNA per molecule of protoxin, indicating that only a small region of the N-terminal toxic moiety interacts with the DNA. It is proposed that the DNA-protoxin complex is virus-like in structure with a central DNA core surrounded by protein interacting with the DNA with the peripheral ends of the C-terminal region extending outward. It is shown that this structure accounts for the unusual proteolysis observed in the generation of toxin in which it appears that peptides are removed by obligatory sequential cleavages starting from the C terminus of the protoxin. Activation of the protoxin by spruce budworm (Choristoneura fumiferana) gut juice is shown to proceed through intermediates consisting of protein-DNA complexes. Larval trypsin initially converts the 20-kilobase pair DNA-protoxin complex to a 20-kilobase pair DNA-toxin complex, which is subsequently converted to a 100-ba...Continue Reading

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Citations

Mar 21, 2002·Biochimica Et Biophysica Acta·Luke MassonRoland Brousseau
Jan 24, 2002·Insect Biochemistry and Molecular Biology·Johann P SchernthanerHarvey Kaplan
Nov 18, 2003·Annual Review of Genetics·Ruud A de MaagdH Ernest Schnepf
Feb 1, 2011·FEMS Microbiology Letters·Shuyuan GuoJie Zhang
Feb 15, 2005·Journal of Invertebrate Pathology·A W SmithJ P Allen
Aug 31, 1999·Archives of Insect Biochemistry and Physiology·B Oppert
Feb 2, 2002·Nature Reviews. Molecular Cell Biology·Abraham MinskyDaphna Frenkiel-Krispin
Mar 12, 2017·Toxins·Rooma AdalatAbdul Rauf Shakoori
Sep 14, 2017·Biotechnology & Genetic Engineering Reviews·Jonathan R LathamAngelika Hilbeck
Sep 8, 1998·Microbiology and Molecular Biology Reviews : MMBR·E SchnepfD H Dean

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