Role of domain I of neuronal Ca2+ channel alpha1 subunits in G protein modulation

The Journal of Physiology
G J StephensAnnette C Dolphin

Abstract

1. We studied the G protein inhibition of heteromultimeric neuronal Ca2+ channels by constructing a series of chimeric channels between the strongly modulated alpha1B subunit and the alpha1E(rbEII) subunit, which showed no modulation. 2. In parallel studies, alpha1 subunit constructs were co-expressed together with the accessory Ca2+ channel alpha2-delta and beta2a subunits in mammalian (COS-7) cells and Xenopus oocytes. G protein inhibition of expressed Ca2+ channel currents was induced by co-transfection of Gbeta1 and Ggamma2 subunits in COS-7 cells or activation of co-expressed dopamine (D2) receptors by quinpirole (100 nM) in oocytes. 3. The data indicate that transfer of the alpha1B region containing the N-terminal, domain I and the I-II loop (i.e. the alpha1B1-483 sequence), conferred G protein modulation on alpha1E(rbEII), both in terms of a slowing of activation kinetics and a reduction in current amplitude. 4. In contrast, the data are not consistent with the I-II loop and/or the C-terminal forming a unique site for G protein modulation.

References

Oct 1, 1994·Pflügers Archiv : European journal of physiology·J I Luebke, K Dunlap
Mar 21, 1996·Nature·S HerlitzeW A Catterall
Feb 20, 1996·Proceedings of the National Academy of Sciences of the United States of America·E BourinetT P Snutch
Feb 18, 1997·Proceedings of the National Academy of Sciences of the United States of America·S HerlitzeW A Catterall
Mar 3, 1998·The Journal of Physiology·A C Dolphin
Jun 17, 1998·The Journal of Physiology·G J StephensA C Dolphin

❮ Previous
Next ❯

Citations

Dec 15, 2010·Channels·Kevin P M Currie
Sep 6, 2005·Biochemical and Biophysical Research Communications·R A Bannister, K G Beam
Nov 21, 2007·Acta Physiologica·A P FoxZ Xie
Apr 28, 2011·The Journal of Physiology·Giovanna BucciGary J Stephens
Nov 30, 2006·Pharmacological Reviews·H William Tedford, Gerald W Zamponi
Dec 6, 2003·Pharmacological Reviews·Annette C Dolphin
Feb 1, 2005·American Journal of Physiology. Lung Cellular and Molecular Physiology·J L CarrollL M Sterni
Oct 10, 2002·The European Journal of Neuroscience·Véronique CornetMichel De Waard
Sep 29, 2020·British Journal of Pharmacology·Claire M Williams, Gary J Stephens

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.