Role of DREF in transcriptional regulation of the Drosophila p53 gene

Oncogene
N Trong-TueM Yamaguchi

Abstract

The tumor suppressor protein p53 has a critical role in safeguarding the integrity of the genome. Its functions are well understood but factors responsible for the transcriptional regulation of the p53 gene are almost entirely unknown. The DNA replication-related element (DRE)/DNA replication-related element-binding factor (DREF) transcriptional regulatory system is established as a master key to cell proliferation in Drosophila. DREF binds specifically to DRE sequences in the Drosophila p53 (dmp53) gene promoter as shown using anti-DREF antibodies in chromatin immunoprecipitation assays. Furthermore, a rough eye phenotype because of overexpression of DREF in Drosophila eye imaginal disks could be suppressed by half dose reduction of the dmp53 gene. In addition, the level of mRNA of dmp53 was decreased in DREF-knockdown cells and transient expression of the luciferase gene under control of the wild-type dmp53 gene promoter showed strong promoter activity in S2 cells, but this was almost completely abrogated with a DRE-mutated promoter. Requirement of DREs for dmp53 promoter activity was further confirmed by anti-beta-galactosidase antibody-staining of various tissues from transgenic flies carrying dmp53 promoter-lacZ fusion gen...Continue Reading

References

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Dec 14, 2005·Biochimica Et Biophysica Acta·Koji OkudairaMasamitsu Yamaguchi
Apr 25, 2006·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Hirokazu SetoMasamitsu Yamaguchi
Sep 5, 2006·Biochimica Et Biophysica Acta·Yuko HayashiMasamitsu Yamaguchi
Sep 12, 2006·Experimental Cell Research·Dang Thi Phuong ThaoMasamitsu Yamaguchi
Jan 16, 2007·Molecular and Cellular Biology·Daisuke YamashitaFumiko Hirose
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May 31, 2007·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Young Shin KimMi Ae Yoo
Sep 25, 2007·Experimental Cell Research·Hiroyuki IdaMasamitsu Yamaguchi
Dec 25, 2007·Biochimica Et Biophysica Acta·Akio MatsukageMasamitsu Yamaguchi
May 31, 2008·Nucleic Acids Research·Kumi NakamuraMasamitsu Yamaguchi
Jan 20, 2009·Experimental Cell Research·Osamu SuyariMasamitsu Yamaguchi

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Citations

Mar 24, 2011·Molecular Therapy : the Journal of the American Society of Gene Therapy·Phong TrangFrank J Slack
Nov 4, 2010·Nature Reviews. Clinical Oncology·Nicole M A WhiteGeorge M Yousef
Oct 5, 2010·Nature Reviews. Drug Discovery·Ramiro GarzonCarlo M Croce
Jun 27, 2013·Cell Structure and Function·Akihito KawamoriMasamitsu Yamaguchi
Jun 4, 2011·Mitochondrion·Phillippa J CarlingPatrick F Chinnery
Nov 4, 2010·Gene·Nguyen Trong TueMasamitsu Yamaguchi
Nov 27, 2014·Scientific Reports·Nicole VoMasamitsu Yamaguchi
Aug 3, 2013·Genesis : the Journal of Genetics and Development·Akihito KawamoriMasamitsu Yamaguchi
Feb 7, 2012·Genesis : the Journal of Genetics and Development·Yasuhide YoshiokaMasamitsu Yamaguchi

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