Role of E boxes in the repression of E-cadherin expression

Biochemical and Biophysical Research Communications
L A GiroldiJ A Schalken

Abstract

Decreased expression of the intercellular adhesion molecule E-cadherin correlates with tumor aggressiveness and invasion capacity of cell lines. The decrease of E-cadherin expression results primarily from reduced mRNA expression. We show here that the activity of a human E-cadherin promoter construct is cell specific and correlates with E-cadherin mRNA expression. This spectrum of activity is conserved by a region as short as 81 bp obtained after 5' deletion. Mutation analysis revealed that two E box elements of this minimal promoter are involved in the silencing of E-cadherin promoter activity occurring in cancer cells. E boxes are mainly known as target sequences for bHLH transcription factors that are involved in the control of tissue differentiation and are antagonised by HLH proteins. However, mutation data and cotransfection experiments using HLH protein expression vectors indicate that bHLH transcription factors are not significantly involved in the E box mediated silencing of this E-cadherin promoter fragment.

Citations

Aug 24, 2005·Oncogene·Yan-Nan LiuJi Hshiung Chen
Apr 5, 2013·Genetic Testing and Molecular Biomarkers·Nageswara Rao TipirisettiVishnupriya Satti
Oct 12, 2012·Cancer Research·Giovanna Ferrari-AmorottiBruno Calabretta
Mar 8, 2011·Biological Chemistry·Johannes M NuessleAndre Menke
Oct 19, 2013·International Journal of Molecular Sciences·Nathalie Zucchini-PascalRoger Rahmani
Feb 23, 2013·World Journal of Gastroenterology : WJG·Qin-Hua ChenYi-Mei Fan
Jul 31, 2007·Reproduction : the Official Journal of the Society for the Study of Fertility·Kang ShanLi Yan
Jun 3, 2006·Proceedings of the National Academy of Sciences of the United States of America·Qinghong ZhangRichard H Goodman
Aug 26, 2003·Proceedings of the National Academy of Sciences of the United States of America·Tatsuo OhiraHarry A Drabkin
Apr 5, 2003·Proceedings of the National Academy of Sciences of the United States of America·Madeleine GrooteclaesSteven M Frisch
Sep 10, 2014·BioMed Research International·Thian-Sze WongJimmy Yu-Wai Chan
Feb 4, 2016·Acta Biochimica Et Biophysica Sinica·Pan ZhaoXuejun Zhang
Feb 5, 2013·Biochemical and Biophysical Research Communications·Benjamin KoopmanschRosita Winkler
Apr 12, 2002·International Journal of Cancer. Journal International Du Cancer·Björn-Anders JonssonHenrik Grönberg
Aug 31, 2010·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Wenjing ZhangBenjamin C Y Wong
Apr 22, 2009·Biochemical and Biophysical Research Communications·Somesh Baranwal, Suresh K Alahari
Oct 21, 2015·Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology·Kioomars SaliminejadHamid Reza Khorram Khorshid
Sep 6, 2008·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Timothy R McPheeShoukat Dedhar
Aug 29, 2006·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Lambertus A KiemeneyGerald W Verhaegh
Apr 26, 2015·Molecular Immunology·C Rojas-MarquezV Ortiz-Navarrete
Jun 10, 2008·Developmental Cell·Jing Yang, Robert A Weinberg
Dec 17, 2014·Neoplasia : an International Journal for Oncology Research·Giovanna Ferrari-AmorottiBruno Calabretta
Nov 10, 2007·Developmental Biology·E G ColesM Bronner-Fraser
Jul 21, 2016·Pathology Oncology Research : POR·Mohammad Mahdi ForghanifardMohammad Reza Abbaszadegan

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Cadherins and Catenins

Cadherins (named for "calcium-dependent adhesion") are a type of cell adhesion molecule (CAM) that is important in the formation of adherens junctions to bind cells with each other. Catenins are a family of proteins found in complexes with cadherin cell adhesion molecules of animal cells: alpha-catenin can bind to β-catenin and can also bind actin. β-catenin binds the cytoplasmic domain of some cadherins. Discover the latest research on cadherins and catenins here.