Role of EGF receptor activation in angiotensin II-induced renal epithelial cell hypertrophy

Journal of the American Society of Nephrology : JASN
Jianchun ChenRaymond C Harris

Abstract

For determination of the molecular mechanisms underlying the induction of epithelial cell hypertrophy by angiotensin II (Ang II), a well-characterized porcine renal proximal tubular cell line LLCPKcl4, which does not express endogenous Ang II receptor subtypes, was transfected with cDNA encoding Ang II subtype 1 receptor (AT1R/Cl4). Ang II transactivated the EGF receptor (EGFR) in these AT1R/Cl4 cells, which was blocked by the selective AT1R antagonist losartan but not by the selective AT2R antagonist PD123319. Ang II did not transactivate EGFR in empty vector-transfected LLCPKcl4 cells (Vector/Cl4). Ang II elicited release of soluble heparin-binding EGF-like growth factor (HB-EGF) from AT1R/Cl4 cells, and Ang II-induced EGFR activation was prevented by pretreatment with the specific HB-EGF inhibitor CRM197 or the metalloproteinase inhibitors batimastat or phenanthroline, none of which had any effect on EGFR activation by exogenously administered EGF. Ang II stimulated protein synthesis and cell hypertrophy in AT1R/Cl4 cells without increasing cell number, and signaling studies revealed that Ang II stimulated phosphorylation of the 40S ribosomal protein S6 and the eukaryotic translation initiation factor 4E-binding protein 1, t...Continue Reading

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Citations

Oct 8, 2011·Journal of the American Society of Nephrology : JASN·Anna Greka, Peter Mundel
Nov 19, 2011·Journal of the American Society of Nephrology : JASN·Jianchun ChenRaymond C Harris
Jan 14, 2012·Journal of the American Society of Nephrology : JASN·Hirokazu Okada
Jan 8, 2014·Nature Reviews. Nephrology·Jochen ReiserChristian Faul
Nov 8, 2011·Annual Review of Physiology·Anna Greka, Peter Mundel
Oct 15, 2014·Nature Reviews. Nephrology·Helena M KokTri Q Nguyen
Nov 14, 2015·Annual Review of Pharmacology and Toxicology·Steven J ForresterSatoru Eguchi
Mar 27, 2007·The International Journal of Biochemistry & Cell Biology·Veronica A StevensCarol A Pollock
Mar 29, 2012·Experimental Diabetes Research·Meenalakshmi M Mariappan
May 20, 2015·The Journal of Clinical Investigation·Jian-Kang ChenRaymond C Harris
Apr 21, 2009·Pharmacology & Therapeutics·Gopala K RanganKok On Ho
Apr 6, 2016·International Journal of Immunopathology and Pharmacology·A MascaliM Tesauro
May 27, 2016·American Journal of Physiology. Renal Physiology·Fenghua ZengRaymond C Harris
Nov 5, 2008·Hypertension·Wynand B W H MelenhorstHarry van Goor
Jul 2, 2011·Journal of the American Society of Nephrology : JASN·Christiane Rüster, Gunter Wolf
Dec 5, 2014·Molecular Pharmacology·Hiroki SakaiMakoto Inui
Jul 1, 2016·Physiological Reviews·Jianchun ChenRaymond C Harris
Jan 26, 2016·Current Opinion in Nephrology and Hypertension·Lili Zhou, Youhua Liu
Mar 31, 2021·Molecular and Cellular Endocrinology·Fitra RiantoMatthew A Sparks

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