Role of elevated pressure in TRAIL-induced apoptosis in human lung carcinoma cells

Apoptosis : an International Journal on Programmed Cell Death
Sangnam OhEunil Lee

Abstract

TNF-related apoptosis-inducing ligand (TRAIL, Apo2L) is a promising anticancer agent with high specificity for cancer cells. Many strategies have been proposed to enhance the sensitivity of cancer cells to TRAIL-mediated apoptosis, including the use of combination treatment with conventional cancer therapies. However, few reports have evaluated the effects of TRAIL in combination with mechanical stress, which can also cause apoptosis of cancer cells. In the present study, we describe a custom-designed culture system that delivers two atmospheres of elevated pressure (EP) by using compressed air, and which enhances the sensitivity of cancer cells to TRAIL-mediated apoptosis. The combination of TRAIL and EP significantly increased apoptosis of human H460 lung cancer cells more than hyperbaric normoxia or normobaric mild hyperoxia. EP-potentiating TRAIL-mediated apoptosis of H460 cells was accompanied by up-regulated death receptor 5 (DR5), activation of caspases, decreased mitochondrial membrane potential, and reactive oxygen species production. We also observed EP-induced sensitization of TRAIL-mediated apoptosis in other cancer cell types. In contrast, human normal cells showed no DNA damage or cell death when exposed to the co...Continue Reading

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Citations

Jul 21, 2010·Biochemical and Biophysical Research Communications·Sangnam OhEunil Lee
Jul 1, 2015·Cellular & Molecular Biology Letters·Eunyoung HongYongchul Lim
Apr 26, 2018·Biophysical Journal·Jarek WosikMalgorzata Kloc

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