Role of epigenetic modifications in the aberrant CYP19A1 gene expression in polycystic ovary syndrome

Archives of Medical Science : AMS
Elham HosseiniReza Afatoonian

Abstract

In this study, the global DNA methylation, histone acetylation and methylation levels of cumulus cells (CCs) in infertile polycystic ovary syndrome (PCOS) patients and the correlation of these epigenetic modifications with the expression of the ovarian aromatase gene (as an important marker in the etiology of PCOS) were investigated. A cross-sectional study was conducted on 24 patients (12 PCOS patients and 12 healthy women), who underwent ovarian stimulation. Nucleosome ELISA was performed, in order to identify the global occupancy level of Mecp2 (as a marker of DNA methylation) and H3K9me2/H3K9ac as histone modification markers in chromatin fractions obtained from CCs. The CYP19A1 gene expression was measured by qRT-PCR. The level of DNA incorporation of MeCP2, histone modification markers and binding of estrogen receptor β (ERβ) to CYP19A1 regulatory sequences were examined by ChIP-QPCR assay. The data demonstrate a significant increase in global occupancy levels of MeCP2 and H3K9ac markers and a decrease of H3K9me2 to chromatin in CCs of PCOS patients vs. control group. Furthermore, CYP19A1 gene expression, and the incorporation of H3K9ac in PII, PI.3, and PI.4 promoters of CYP19A1 in PCOS, were higher than those of control...Continue Reading

Citations

Dec 12, 2020·Clinical Obstetrics and Gynecology·Joshua C CombsAlan H Decherney
Feb 7, 2021·European Journal of Medical Genetics·Isaac Kyei Barffour, Roselind Kyei Baah Kwarkoh
Apr 8, 2021·Reproductive Sciences·Matheus Credendio EirasCristiana Libardi Miranda Furtado
Aug 20, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Chang-Zhu PeiKwang-Hyun Baek

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Methods Mentioned

BETA
acetylation
ELISA
immunoprecipitation
PCR
histone acetylation
ChIP

Software Mentioned

SPSS Statistics

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