Role of erythrocytes and platelets in the hypercoagulable status in polycythemia vera through phosphatidylserine exposure and microparticle generation

Thrombosis and Haemostasis
Xiaoyan TanJin Zhou

Abstract

The development of thrombosis in polycythaemia vera (PV) involves multifactorial processes including pathological activation of blood cells. Release of microparticles (MPs) by activated cells in diseases is associated with thrombotic risk, but relatively few data are available in PV. The aim of the present study was to investigate the increase in MP release and exposure of phosphatidylserine (PS) on the outer membrane of MP-origin cells in patients with PV, and to analyse their procoagulant activity (PCA). PS-positive MPs and cells were detected by flow cytometry, while PCA was assessed with clotting time and purified coagulation complex assays. We found that PV patients had elevated circulating lactadherin+ MPs, which mostly originating from erythrocytes, platelets, granulocytes, and endothelial cells, as well as increased PS exposing erythrocytes/platelets as compared to secondary polycythaemia patients or healthy controls. These PS-bearing MPs and cells were highly procoagulant. Moreover, lactadherin competed factor V and VIII to PS and inhibited about 90% of the detected PCA in a dose-response manner while anti-TF antibody did no significant inhibition. Treatment with hydroxyurea is associated with a decrease in PS exposure...Continue Reading

References

Jul 4, 2013·Annals of Hematology·Armando TripodiFlora Peyvandi
Mar 7, 2014·Antioxidants & Redox Signaling·Donatella PietraforteElisabetta Straface
May 29, 2014·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·Kewei Wang
Jul 16, 2015·Journal of Thrombosis and Haemostasis : JTH·B ØsterudV Y Bogdanov
Jan 13, 2015·Blood Reviews·Michael H KrollSrdan Verstovsek
Dec 25, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Chunfa YangXiaoming Zou
Feb 24, 2015·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Micah J Mooberry, Nigel S Key
Mar 27, 2016·Journal of Experimental & Clinical Cancer Research : CR·Liangliang ZhaoDaxun Piao
Apr 15, 2015·Journal of Veterinary Internal Medicine·L KiddN Mackman
Jul 19, 2016·Indian Journal of Hematology & Blood Transfusion : an Official Journal of Indian Society of Hematology and Blood Transfusion·Hara Prasad Pati, Prashant Sharma
May 21, 2016·Liver International : Official Journal of the International Association for the Study of the Liver·Xiaoming WuJialan Shi
Mar 13, 2018·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Muxin YuJialan Shi
Jan 19, 2019·Cancers·Caroline J ReddelVivien M Chen
Feb 12, 2020·The Journal of Clinical Investigation·Johanne PoissonPierre-Emmanuel Rautou
Jun 8, 2018·Biotechnology Letters·Jessica ThomasNicanor I Moldovan

Citations

Sep 15, 1996·European Journal of Biochemistry·J HvarregaardT E Petersen
May 7, 2002·Clinical and Applied Thrombosis/hemostasis : Official Journal of the International Academy of Clinical and Applied Thrombosis/Hemostasis·Katya PresseizenMartin H Ellis
Oct 24, 2002·Blood·Jerry L Spivak
Nov 10, 2004·Biochimica Et Biophysica Acta·Jialan ShiG E Gilbert
Nov 24, 2006·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Jialan ShiG E Gilbert
Dec 13, 2006·British Journal of Haematology·Marcello Di NisioEuropean Collaboration on Low-dose Aspirin in Polycythemia Vera (ECLAP) Investigators
Nov 13, 2007·American Journal of Physiology. Heart and Circulatory Physiology·Johnson HaynesTroy Stevens
Sep 19, 2008·Leukemia·Raffaele LandolfiAnna Falanga
May 14, 2009·Platelets·F Dignat-GeorgeNigel S Key
Feb 13, 2010·Thrombosis Research·B Østerud
Mar 3, 2010·Annual Review of Biophysics·Peter A Leventis, Sergio Grinstein
Oct 19, 2010·British Journal of Haematology·Hiroshi FujitaShigeko Nishimura
Oct 30, 2010·Circulation Research·Sebastian F Mause, Christian Weber
Nov 9, 2010·Thrombosis and Haemostasis·Johannes J A AuwerdaFrank W G Leebeek
Dec 17, 2010·Arteriosclerosis, Thrombosis, and Vascular Biology·Olivier MorelFlorence Toti
Dec 22, 2010·Thrombosis and Haemostasis·Yuana YuanaSusanne Osanto
Jun 22, 2011·Basic Research in Cardiology·Silvia Montoro-GarcíaGregory Y H Lip
Jun 30, 2011·Thrombosis and Haemostasis·Gala TsimermanAnat Aharon
Jan 14, 2012·Clinical Hemorheology and Microcirculation·Rosalia Lo PrestiGregorio Caimi
Apr 25, 2012·Hematology·Raffaele Landolfi, Leonardo Di Gennaro

Related Concepts

Polycythemia Vera
Flow Cytometry
Blood Microparticle Formation
Neutrophil Band Cells
Thrombophilia
Outer Membrane
Phosphatidylserines
Factor V
Coagulants
Granulocyte Count

Related Feeds

Blood Clotting Disorders

Thrombophilia includes conditions with increased tendency for excessive blood clotting. Blood clotting occurs when the body has insufficient amounts of specialized proteins that make blood clot and stop bleeding. Here is the latest research on blood clotting disorders.