Role of Escherichia coli nitrogen regulatory genes in the nitrogen response of the Azotobacter vinelandii NifL-NifA complex

Journal of Bacteriology
Francisca Reyes-RamirezR Dixon

Abstract

The redox-sensing flavoprotein NifL inhibits the activity of the nitrogen fixation (nif)-specific transcriptional activator NifA in Azotobacter vinelandii in response to molecular oxygen and fixed nitrogen. Although the mechanism whereby the A. vinelandii NifL-NifA system responds to fixed nitrogen in vivo is unknown, the glnK gene, which encodes a PII-like signal transduction protein, has been implicated in nitrogen control. However, the precise function of A. vinelandii glnK in this response is difficult to establish because of the essential nature of this gene. We have shown previously that A. vinelandii NifL is able to respond to fixed nitrogen to control NifA activity when expressed in Escherichia coli. In this study, we investigated the role of the E. coli PII-like signal transduction proteins in nitrogen control of the A. vinelandii NifL-NifA regulatory system in vivo. In contrast to recent findings with Klebsiella pneumoniae NifL, our results indicate that neither the E. coli PII nor GlnK protein is required to relieve inhibition by A. vinelandii NifL under nitrogen-limiting conditions. Moreover, disruption of both the E. coli glnB and ntrC genes resulted in a complete loss of nitrogen regulation of NifA activity by Nif...Continue Reading

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·G Pahel, B Tyler
Oct 1, 1995·FEMS Microbiology Letters·W C van HeeswijkH V Westerhoff
Apr 20, 1995·Molecular & General Genetics : MGG·R Edwards, M Merrick
Aug 1, 1993·Molecular Microbiology·G BlancoC Kennedy
Dec 1, 1995·Microbiological Reviews·M J Merrick, R A Edwards
Jul 1, 1996·Molecular Microbiology·W C van HeeswijkH V Westerhoff
Jun 11, 1998·Journal of Bacteriology·D MeletzusC Kennedy
May 7, 1999·Molecular Microbiology·M R Atkinson, A J Ninfa
Jan 19, 2000·Trends in Genetics : TIG·G ThomasM Merrick
Feb 7, 2001·Trends in Microbiology·A J Ninfa, M R Atkinson
Jul 14, 2000·Journal of Bacteriology·M BuckJ D Gralla
Aug 10, 2000·Molecular Microbiology·G H ThomasM Merrick

❮ Previous
Next ❯

Citations

Jul 20, 2010·The Journal of Biological Chemistry·Martha V RadchenkoMike Merrick
Aug 5, 2004·European Journal of Biochemistry·Jessica StipsRuth A Schmitz
Jan 16, 2002·Journal of Bacteriology·Paul RudnickChristina Kennedy
Mar 6, 2003·Journal of Bacteriology·David J Studholme, Ray Dixon
Dec 13, 2005·Microbiology and Molecular Biology Reviews : MMBR·Ravi D Barabote, Milton H Saier
Nov 10, 2004·Proceedings of the National Academy of Sciences of the United States of America·Isabel Martinez-ArgudoRay Dixon
Jun 30, 2005·Proceedings of the National Academy of Sciences of the United States of America·Sophie LaurentCheng-Cai Zhang
Feb 11, 2005·Journal of Molecular Biology·Susan PerryRay Dixon
Aug 7, 2012·FEMS Microbiology Reviews·Luciano F HuergoMike Merrick
Oct 2, 2015·Microbiology and Molecular Biology Reviews : MMBR·Luciano F Huergo, Ray Dixon
Dec 12, 2003·The Journal of Biological Chemistry·Arnaud JavelleMike Merrick
Jan 26, 2005·Biochemical Society Transactions·I Martinez-ArgudoR Dixon
Mar 16, 2007·The Journal of Biological Chemistry·Richard LittleRay Dixon
Dec 7, 2018·Archives of Microbiology·Thiago Estefano RodriguesLuciano Fernandes Huergo

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.