Role of estrogen receptor-alpha and -beta in regulating leptin expression in 3T3-L1 adipocytes

Obesity
Kyong Wook YiJun-Young Hur

Abstract

We investigated the effects of the estrogen receptor-alpha (ERalpha) and -beta (ERbeta) in the regulation of leptin, resistin, and adiponectin expression in 3T3-L1 adipocytes. Mature adipocytes were exposed to estradiol (E2), ERalpha agonist (PPT (4,4',4''-(4-propyl-[1H]-pyrazole-1,3,5-triyl)trisphenol)), ERbeta agonist (DPN (2,3-bis(4-Hydroxyphenyl)-propionitrile)), E2 with ERalpha antagonist (MPP (1,3-Bis(4-hydroxyphenyl)-4-methyl-5-[4-(2-piperidinylethoxy)phenol]-1H-pyrazole dihydrochloride)), and E2 with ERbeta antagonist (R,R-THC ((R,R)-5,11-diethyl-5,6,11,12-tetrahydro-2,8-chrysenediol)) at different concentrations. To clarify the expression and regulation of adipokines by ER subtypes, total RNA was extracted from cells and measured using quantitative PCR. Western blot analysis was performed to evaluate the protein expression of adipokines, ERalpha, and ERbeta. The leptin expression was significantly increased in the cells treated with high concentrations (10(-5) and 10(-6) mol/l) of the PPT (P < 0.01, P < 0.05). By contrast, the leptin expression decreased in a dose-dependent manner in the MPP-treated groups (P < 0.05). High concentrations (10(-5) mol/l) of R,R-THC with E2 (10(-7) mol/l) caused a significant increase of ...Continue Reading

References

Feb 1, 1996·The New England Journal of Medicine·R V ConsidineT L Bauer
Jun 11, 1996·Proceedings of the National Academy of Sciences of the United States of America·G G KuiperJ A Gustafsson
Aug 1, 1997·The Journal of Endocrinology·H ShimizuM Mori
Jul 11, 1998·Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Métabolisme·N YonedaT Aono
Oct 31, 1998·Nature·J M Friedman, J L Halaas
Mar 27, 1999·Biochemical and Biophysical Research Communications·Y AritaY Matsuzawa
May 7, 1999·European Journal of Endocrinology·L PinillaC Dieguez
May 21, 1999·Biochemical and Biophysical Research Communications·H Watanobe, T Suda
Jun 12, 1999·Biochemical and Biophysical Research Communications·K KristensenB Richelsen
Jun 10, 2000·Arteriosclerosis, Thrombosis, and Vascular Biology·K HottaY Matsuzawa
Dec 29, 2000·International Journal of Obesity and Related Metabolic Disorders : Journal of the International Association for the Study of Obesity·R ZechnerS Levak-Frank
Feb 24, 2001·Nature·C M SteppanM A Lazar
May 24, 2001·Proceedings of the National Academy of Sciences of the United States of America·Z WeihuaJ A Gustafsson
Sep 22, 2001·The Journal of Steroid Biochemistry and Molecular Biology·S MaruyamaA Ito
May 3, 2002·The Journal of Biological Chemistry·Meng-Min LiuPeter J Kushner
May 8, 2002·The Journal of Clinical Endocrinology and Metabolism·Philip G McTernanSudhesh Kumar
Mar 11, 2003·Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology·C Di CarloC Nappi
Mar 14, 2003·Obesity Research·Rubén NogueirasCarlos Diéguez
Mar 28, 2003·Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Métabolisme·A NaazP S Cooke
Nov 29, 2005·Metabolism: Clinical and Experimental·Lucyna SieminskaMariusz Nowak
May 26, 2006·Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology·Grazyna Bednarek-TupikowskaAndrzej Milewicz
Dec 14, 2006·American Journal of Physiology. Endocrinology and Metabolism·John W BullenChristos S Mantzoros

❮ Previous
Next ❯

Citations

Jun 11, 2010·European Journal of Cancer Prevention : the Official Journal of the European Cancer Prevention Organisation (ECP)·Graeme P Williams
Feb 24, 2015·Frontiers in Oncology·Francesco CaiazzaKieran Sheahan
Jun 9, 2016·International Journal of Obesity : Journal of the International Association for the Study of Obesity·S Ahmed, E Atlas
Dec 23, 2017·Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology·Nazish RafiqueMona H ALSheikh
Apr 25, 2012·Cytotechnology·Kazem Nejati-KoshkiHamid Tayefi-Nasrabadi
Jul 17, 2013·International Journal of Molecular Medicine·Un Suk JungJun-Young Hur
Jan 1, 2014·Asian Pacific Journal of Cancer Prevention : APJCP·Kazem Nejati-KoshkiHassan Dariushnejad
Dec 24, 2019·Annals of the New York Academy of Sciences·John R BrachtDeborah J Clegg
Jan 1, 2014·Cancer Cell International·Kazem Nejati-KoshkiMohammad Pourhassan-Moghaddam
Nov 20, 2016·Journal of Cellular and Molecular Medicine·Kimberly A Cox-YorkRachael E Van Pelt
May 28, 2020·International Journal of Molecular Sciences·Michal DulibanBarbara Bilinska
Sep 27, 2018·The Journal of Steroid Biochemistry and Molecular Biology·Marco Bauzá-ThorbrüggeAna M Proenza
Mar 25, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Seema PatelBiswa Ranjan Meher

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.