PMID: 7535855Dec 1, 1994Paper

Role of F-actin in the activation of Na(+)-K(+)-Cl- cotransport by forskolin and vasopressin in mouse kidney cultured thick ascending limb cells

The Journal of Membrane Biology
M S WuA Vandewalle

Abstract

The influence of microtubules and F-actin on Na(+)-K(+)-Cl- cotransport was investigated in cultured cells derived from outer-medullary thick ascending limb tubules microdissected from the mouse kidney. The cultured cells contained Tamm-Horsfall protein, produced cAMP in response to dD-arginine vasopressin (dD-AVP), isoproterenol, prostaglandin E2 and forskolin (FK), and exhibited an ouabain-resistant furosemide-sensitive (Or-Fs) component of 86Rb+ influx mediated by the Na(+)-K(+)-Cl- cotransporter. Both FK and dD-AVP stimulated the Or-Fs component of Rb+ influx. Neither agent altered the tubulin and cytokeratin networks nor the shape of the tight junction using a specific anti-ZO-1 antibody. In contrast, they did induce a marked redistribution of F-actin to the periphery of the cells delineating the tight junctions. Preincubation of the cells with nocodazole, to disrupt microtubules, did not alter the FK- or dD-AVP-elicited Or-Fs Rb+ influx. In contrast, phalloidin and NBD-phallicidin, which stabilize F-actin, markedly impaired the stimulation of Na(+)-K(+)-Cl- cotransport by FK or dD-AVP, without affecting the Na(+)-K+ ATPase pumps and the rate constant of 36Cl- and 86Rb+ efflux. These results strongly suggested that cAMP-st...Continue Reading

Citations

Apr 23, 2008·Pflügers Archiv : European journal of physiology·M Bens, A Vandewalle
Jul 20, 2012·Cell Biochemistry and Biophysics·Arpita Kundu, Anbarasu Anand
Dec 20, 2000·Transplantation Proceedings·S T PangA Vandewalle
Dec 9, 2000·Transplant International : Official Journal of the European Society for Organ Transplantation·M S WuA Vandewalle
Mar 18, 2011·American Journal of Physiology. Cell Physiology·Winnie Waichi ShumSylvie Breton
Feb 1, 2005·Cerebrospinal Fluid Research·Conrad JohansonEdward Stopa
Jul 28, 2013·Pflügers Archiv : European journal of physiology·Bob GlaudemansOlivier Devuyst
Jan 20, 1999·Kidney International·N H GarcíaJ L Garvin
Mar 29, 2002·Kidney International·Jérôme NicodPaolo Ferrari
Oct 19, 1999·Microscopy Research and Technique·J H Henson
Jul 25, 1998·FEBS Letters·P ManuntaG Bianchi
Jan 23, 2003·The Journal of Pharmacology and Experimental Therapeutics·Tong Wang
Dec 28, 2007·American Journal of Physiology. Cell Physiology·Núria M Pastor-SolerSylvie Breton
Dec 29, 1995·The Journal of Biological Chemistry·K SteinmeyerT J Jentsch
Sep 25, 2004·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Mai-Szu WuYung-Chih Chen
Aug 11, 2020·Journal of Cellular Physiology·Andree-Anne MarcouxPaul Isenring
May 2, 2003·American Journal of Physiology. Renal Physiology·Mauro BustamanteEric Feraille
Oct 12, 1999·The American Journal of Physiology·M FerrandiS J Karlish
Sep 13, 2007·The Journal of Biological Chemistry·Boubacar BenzianeKamel Laghmani
Dec 14, 2004·The Journal of Biological Chemistry·Valérie BeaulieuSylvie Breton
Aug 1, 1995·Current Opinion in Cell Biology·J A Payne, B Forbush

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.