Role of FK506-binding protein 12 in development of the chick embryonic heart

Biochemical and Biophysical Research Communications
K ObataM Yokota

Abstract

A cDNA encoding chicken FK506-binding protein 12 (FKBP12) was isolated and sequenced. The predicted amino acid sequence of the chicken protein shows high homology to those of FKBP12 proteins of other species ranging from human to frog. The possible role of FKBP12 in chick embryonic cardiac development was examined. Northern blot analysis revealed that FKBP12 mRNA is distributed widely in chick embryos, being especially abundant in the heart; the amount of FKBP12 mRNA in the embryonic heart decreased with time. Administration of FK506 to chick embryos at 7 to 9 days resulted in marked cardiac enlargement. FK506 also reduced the expression of myosin, induced a more elongated cell morphology, and impaired network formation in cultured chick embryonic cardiomyocytes. These results suggest that FKBP12 is important in the regulation of contractile function and phenotypic expression in chick cardiomyocytes during embryonic development.

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Citations

Jan 1, 2003·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Shigenobu YazawaTamao Ono
May 29, 2003·Birth Defects Research. Part C, Embryo Today : Reviews·Joey V Barnett, Jay S Desgrosellier
Sep 27, 2003·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Shigenobu YazawaTamao Ono

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