PMID: 9426700Jan 14, 1998Paper

Role of folate receptor and reduced folate carrier in the transport of 5-methyltetrahydrofolic acid in human ovarian carcinoma cells

International Journal of Cancer. Journal International Du Cancer
G CoronaM Boiocchi

Abstract

Folate receptor-alpha (FR-alpha) is generally over-expressed in non-mucinous human ovarian carcinomas. The meaning of FR-alpha over-expression and its role in the 5-methyltetrahydrofolic acid (N5-CH3-H4PteGlu) transport in such tumors is not clear, especially compared with the reduced folate carrier (RFC), the other known folate transporter. In this study, we analyzed molecular FR-alpha and RFC expression in 16 ovarian carcinoma tissues and in 5 ovarian carcinoma cell lines using competitive PCR. Co-expression of the 2 transporters was found both in vivo and in vitro. FR-alpha mRNA expression in the cell lines was in good agreement with the corresponding protein expression evaluated by cellular folic acid binding and immunofluorescence analysis, using a specific monoclonal antibody (MAb) (MOv18). Moreover, RFC mRNA expression levels were consistent with the selective cellular binding of N-hydroxysuccinimide of [3H]-methotrexate (NHS-MTX). The 5 ovarian carcinoma cell lines (IGROV-1, SW-626, SKOV-3, OVCAR-3 and OAW-42), grown at physiological N5-CH3-H4PteGlu concentrations (20 nM) and expressing FR-alpha and RFC levels superimposable to those observed in vivo, were used as in vitro cellular model to evaluate the different contri...Continue Reading

References

Jul 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·H MatsueS W Lacey
Apr 1, 1991·The Journal of Clinical Investigation·B A KamenR G Anderson
Jan 1, 1990·Annual Review of Nutrition·G B Henderson
Nov 3, 1995·International Journal of Cancer. Journal International Du Cancer·S MiottiS Canevari
Jan 1, 1996·The Journal of Cell Biology·S RijnbouttG J Strous
Apr 22, 1997·International Journal of Cancer. Journal International Du Cancer·G ToffoliM Boiocchi

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Citations

Apr 1, 2009·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·David S MillerCecelia H Boardman
Jun 4, 2011·Biomacromolecules·Danielle S W BenoitPatrick S Stayton
Jul 14, 2001·Critical Reviews in Clinical Laboratory Sciences·J G Donnelly
Feb 14, 2012·Expert Opinion on Investigational Drugs·M MorottiS Ferrero
Dec 10, 2013·Expert Opinion on Biological Therapy·Giuseppe BronteAntonio Russo
Apr 7, 2015·Cellular Signalling·Mariann F HansenPia M Martensen
Mar 7, 2009·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Jonathan A Ledermann, Justin Stebbing
Jan 27, 2009·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Ignace VergoteJalid Sehouli
Jan 22, 2008·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·J LutenW E Hennink
Feb 3, 2006·International Journal of Cancer. Journal International Du Cancer·Linda E Kelemen
Jul 9, 2009·International Journal of Cancer. Journal International Du Cancer·Joanne KotsopoulosShelley S Tworoger
Aug 29, 2014·Molecular Pharmaceutics·Bindu VarghesePhilip S Low
Jan 8, 2010·Cancer Chemotherapy and Pharmacology·Jalid SehouliGuelten Oskay-Oezcelik
Feb 21, 2018·Artificial Cells, Nanomedicine, and Biotechnology·Güliz AkSenay Hamarat Sanlier
Jan 30, 2004·The Journal of Nutrition·Michele M Doucette, Victoria L Stevens
Sep 3, 2010·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Tammy L KalberJimmy D Bell
Feb 6, 2017·British Journal of Cancer·Mariann Fagernæs HansenPia M Martensen
Dec 9, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Shrikanta ChattopadhyayI David Goldman
Jul 10, 2018·Oncology Reviews·Michael RocheJoshua P Kesterson
Jul 13, 2016·Developmental Cell·Snehal N ChaudhariEdward T Kipreos
Jul 2, 1999·Comparative Biochemistry and Physiology. Part C, Pharmacology, Toxicology & Endocrinology·T V AuA J Clifford
Mar 6, 2003·Journal of Controlled Release : Official Journal of the Controlled Release Society·J H van SteenisW E Hennink
Sep 23, 2003·Journal of Controlled Release : Official Journal of the Controlled Release Society·Justin M SaulRavi V Bellamkonda
May 27, 2005·Journal of Medicinal Chemistry·Thommey P ThomasJames R Baker

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