PMID: 1188150Jan 11, 1975Paper

Role of free calcium and ATP in calcium release from cardiac sarcoplasmic reticulum fragments.

Recent Advances in Studies on Cardiac Structure and Metabolism
H R Besch, A M Watanabe

Abstract

Using both isotopic and spectrophotometric techniques to monitor the overall Ca++ binding and release reactions, we have studied the effects of the relative free concentrations of Ca++, sarcoplasmic reticulum fragments (SRF) protein, and ATP on spontaneous Ca++ release by SRF isolated from normal canine hearts. Low free [Ca++] was achieved by using a Ca-EGTA buffer; higher (micromolar) values were obtained by direct Ca++ additions throughout the reactions. ATP concentrations were stabilized by an ATP-regenerating system or an ATP trap. Concentrations of nucleotides in the reaction mixtures were also determined. Release depended in part on the [Ca++]/[ATP] and on the [ATP]. Spontaneous Ca++ release was prevented or slowed when the [ATP] was rapidly lowered with the ATP trap. ATP regeneration delayed, but did not prevent Ca++ release. Ca++-induced Ca++ release was found in the presence of ADP and ATP. Verapamil and D 600 did not affect release. The results may provide a basis for the slowed Ca++ release in SRF from failing hearts.

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.