Role of free radicals and metal ions in direct current-induced cytotoxicity

Journal of Endodontics
Yuko NakamuraHirofumi Nishikawa

Abstract

The purpose of this study was to investigate the mechanism of direct current (DC)-induced cytotoxicity. To test the working hypothesis that electrolysis products are responsible for the DC-induced cytotoxicity, the cytotoxic effects between the direct and indirect DC treatment against human polymorphonuclear cells (PMNs) was compared. The indirect DC treatment (treatment with the culture medium exposed to DC) was comparable in cytotoxicity to the direct DC treatment, suggesting that electrolysis products have an important role in DC-induced cytotoxicity. Metal ions released from different electrodes into the culture medium were quantified by the inductively coupled plasma-mass spectroscopy. Higher concentrations of Ag, Zn, and Ni and chromium were released from Ag, Zn, and stainless steel (St) electrodes, respectively, whereas much lower concentrations of Ni and Ti were released from Ni-Ti electrode. Further, electron spin resonance spectroscopy with spin-trapping agent showed that the direct current with the following metal electrodes generated alkoxyl radical (St and Ni-Ti electrodes), hydrogen radical (Ag and Au electrodes), and both carbon and alkoxyl radicals (Zn electrode), respectively. These results suggest that free ra...Continue Reading

References

Feb 1, 1976·Antimicrobial Agents and Chemotherapy·T J BergerR O Becker
Nov 1, 1976·Antimicrobial Agents and Chemotherapy·T J BergerR O Becker
Nov 1, 1992·Antimicrobial Agents and Chemotherapy·C P DavisM M Warren
Jan 1, 1992·Biomaterials·T HanawaM Ota
Oct 1, 1991·Antimicrobial Agents and Chemotherapy·C P DavisM M Warren
Sep 1, 1991·Journal of Biomedical Materials Research·J C WatahaR G Craig
Sep 1, 1994·Reproductive Toxicology·M RajasekaranS C Sikka
Jul 1, 1996·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·I Fagerheim, E Lingaas
Jan 1, 1996·Bioelectromagnetics·R Goldman, S Pollack
Jun 1, 1997·Endodontics & Dental Traumatology·S W DahlstromT E Bridges
Feb 6, 2004·Journal of Endodontics·Kohji Kawamoto, Yasuhisa Tsujimoto

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Citations

May 24, 2008·Journal of Endodontics·Yuko NakamuraKeiso Takahashi
Jun 16, 2010·Archives of Gynecology and Obstetrics·Imran A SiddiquiHanan M F Al Kadri
Jul 6, 2014·Journal of Neurochemistry·Cécile BatandierFrédéric Canini

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