Role of Free Thiol on Protein Adsorption to Gold Nanoparticles.

Langmuir : the ACS Journal of Surfaces and Colloids
Olatunde AwotundeJeremy D Driskell

Abstract

Protein-gold nanoparticle (AuNP) bioconjugates have many potential applications in nanomedicine. A thorough understanding of the interaction between the protein and the AuNP is critical to engineering these functional bioconjugates with desirable properties. In this work, we investigate the role of free thiols presented by the protein on the stability of the protein-AuNP conjugate. Human serum albumin (HSA) was modified with 2-iminothiolane (Traut's reagent) to introduce additional thiols onto the protein surface, and three variants of HSA were synthesized to present 1, 5, and 20 free thiols by controlling the molar excess of the chemical modifier. Protein exchange studies on AuNPs were conducted using these HSA species and an IgG antibody which exhibited 10 free thiols. Antibody-AuNP conjugates were synthesized, purified, and dispersed in solutions containing each of the HSA species. No protein exchange was detected with the HSA or modified HSA containing 5 thiols; however, 85% of the antibody was displaced on the AuNP surface by the extensively thiolated HSA presenting 20 free thiols. Furthermore, the impact of the protein adsorption sequence was probed in which each of the HSA species were preadsorbed onto the AuNP and dispe...Continue Reading

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Citations

Aug 30, 2020·Nanomaterials·Kyle Bromma, Devika B Chithrani
Feb 24, 2021·Langmuir : the ACS Journal of Surfaces and Colloids·Samuel OkyemJeremy D Driskell
Jun 5, 2021·Mikrochimica Acta·Amandeep KaurNirmal Prabhakar
Jun 24, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Bicong ZhouChangmin Yu

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