Role of G3BP1 in glucocorticoid receptor-mediated microRNA-15b and microRNA-23a biogenesis in endothelial cells

Cellular and Molecular Life Sciences : CMLS
Hoi-Hin KwokRicky Ngok-Shun Wong

Abstract

MicroRNAs (miRNAs) are a family of non-coding RNAs that play crucial roles in regulating various normal cellular responses. Recent studies revealed that the canonical miRNA biogenesis pathway is subject to sophisticated regulation. Hormonal control of miRNA biogenesis by androgen and estrogen has been demonstrated, but the direct effects of the glucocorticoid receptor (GR) on miRNA biogenesis are unknown. This study revealed the role of GR in miRNA maturation. We showed that two GR agonists, dexamethasone and ginsenoside-Rg1 rapidly suppressed the expression of mature miR-15b, miR-23a, and miR-214 in human endothelial cells. RNA pulldown coupled with proteomic analysis identified GTPase-activating protein (SH3 domain) binding protein 1 (G3BP1) as one of the RNA-binding proteins mediating GR-regulated miRNA maturation. Activated GR induced phosphorylation of v-AKT Murine Thymoma Viral Oncogene Homologue (AKT) kinase, which in turn phosphorylated and promoted nuclear translocation of G3BP1. The nuclear G3BP1 bound to the G3BP1 consensus sequence located on primary miR-15b~16-2 and miR-23a~27a~24-2 to inhibit their maturation. The findings from this study have advanced our understanding of the non-genomic effects of GR in the vasc...Continue Reading

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Citations

Jul 4, 2018·American Journal of Physiology. Endocrinology and Metabolism·Ana Isabel MartínAsunción López-Calderón
Aug 24, 2018·Nature Communications·Pabitra K SahooJeffery L Twiss
Apr 25, 2019·The Journal of Endocrinology·Rui SongLubo Zhang
May 28, 2021·Frontiers in Cell and Developmental Biology·Fabian Vega-TapiaLoreto Cuitino

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Methods Mentioned

BETA
nuclear translocation
transfection
PCR
pulldown
protein assay
immunoprecipitation
electrophoretic
in
electrophoresis
Assay

Software Mentioned

Global Alignment of BLAST
Mfold

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