Role of gangliosides in the association of ErbB2 with lipid rafts in mammary epithelial HC11 cells

The FEBS Journal
Elena SottocornolaMaurizio Sorice

Abstract

We analyzed the role of gangliosides in the association of the ErbB2 receptor tyrosine-kinase (RTK) with lipid rafts in mammary epithelial HC11 cells. Scanning confocal microscopy experiments revealed a strict ErbB2-GM3 colocalization in wild-type cells. In addition, analysis of membrane fractions obtained using a linear sucrose gradient showed that ErbB2, epidermal growth factor receptor (EGFR) and Shc-p66 (proteins correlated with the ErbB2 signal transduction pathway) were preferentially enriched in lipid rafts together with gangliosides. Blocking of endogenous ganglioside synthesis by (+/-)-threo-1-phenyl-2-decanoylamino-3-morpholino-1-propanol hydrochloride ([D]-PDMP) induced a drastic cell-surface redistribution of ErbB2, EGFR and Shc-p66, within the Triton-soluble fractions, as revealed by linear sucrose-gradient analysis. This redistribution was partially reverted when exogenous GM3 was added to ganglioside-depleted HC11 cells. The results point out the key role of ganglioside GM3 in retaining ErbB2 and signal-transduction-correlated proteins in lipid rafts.

References

Oct 1, 1992·Experimental Cell Research·L V LottiM R Torrisi
Jan 18, 1980·Biochimica Et Biophysica Acta·L Svennerholm, P Fredman
Jul 1, 1980·Journal of Neurochemistry·M A Williams, R H McCluer
Feb 15, 1994·Proceedings of the National Academy of Sciences of the United States of America·X QianM I Greene
Dec 30, 1994·Biochimica Et Biophysica Acta·N E Hynes, D F Stern
Aug 2, 1996·The Journal of Biological Chemistry·M VecchiG Carpenter
Jun 5, 1997·Nature·K Simons, E Ikonen
Oct 16, 1999·The Journal of Biological Chemistry·C MineoR G Anderson
Jul 17, 2001·Current Opinion in Cell Biology·E Ikonen
Jan 18, 2002·The Journal of Biological Chemistry·Erik A MiljanEric G Bremer
Mar 12, 2002·The Journal of Biological Chemistry·Kirstine RoepstorffBo van Deurs
May 23, 2002·The Journal of Biological Chemistry·Montarop Yamabhai, Richard G W Anderson
Oct 2, 2002·The Journal of Biological Chemistry·Xiao-Qi WangAmy S Paller
Jan 27, 2004·Molecular Biology of the Cell·Anette M HommelgaardBo van Deurs
Jun 9, 2004·European Journal of Biochemistry·Adolfo R ZuritaJosé L Daniotti
Mar 18, 2005·Molecular Biology of the Cell·Claudia PuriCarlo Tacchetti

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Citations

Jan 4, 2008·Breast Cancer Research : BCR·Yuchao GuWengong Yu
Mar 28, 2009·International Journal of Molecular Sciences·Masahito ShimizuHisataka Moriwaki
Jun 25, 2013·Oncogene·V NovitskayaF Berditchevski
Jun 21, 2014·Biochimica Et Biophysica Acta·Fabio Dall'OlioMariella Chiricolo
Apr 25, 2007·Molecular Membrane Biology·Denys MarushchakLennart B-A Johansson
Oct 20, 2009·FEBS Letters·Gerrit van Meer, Sandra Hoetzl
Mar 9, 2010·Brain Research Bulletin·Cara-Lynne Schengrund
Apr 7, 2009·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Yi-He LingYiyu Zou
Nov 21, 2015·Experimental Cell Research·Vincenzo MatteiTina Garofalo
Dec 3, 2014·Biochimica Et Biophysica Acta·Maxime GuéguinouChristophe Vandier
Mar 10, 2010·Cell Adhesion & Migration·Florian Cymer, Dirk Schneider
Feb 22, 2018·International Journal of Molecular Sciences·Inês Gomes FerreiraFabio Dall'Olio
Mar 1, 2008·Neurochemical Research·Kimberly A Petro, Cara-Lynne Schengrund
Sep 8, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Lydie CrescenceEric Mas
May 24, 2019·Journal of Experimental & Clinical Cancer Research : CR·Xiao-Xin ZhangZhi-Gang Zhang

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