PMID: 9171427Jun 1, 1997Paper

Role of GATA factor Nil2p in nitrogen regulation of gene expression in Saccharomyces cerevisiae

Journal of Bacteriology
D W RowenB Magasanik

Abstract

We have identified the product of the NIL2 gene of Saccharomyces cerevisiae which contains a zinc finger region highly homologous to those of the GATA factors Gln3p and Nil1p as an antagonist of Nil1p and to a lesser extent of Gln3p. The expression of many nitrogen-regulated genes of Saccharomyces cerevisiae requires activation by GATA factor Gln3p or Nil1p and is prevented by the presence of glutamine in the growth medium. Disruption of NIL2 results in a great increase in the expression of NIL1 and of GAP1, the structural gene for the general amino acid permease, in glutamine-grown cells in response to activation by Nil1p. The primary effect of the elimination of Nil2p appears to be an increase in the intracellular level of Nil1p, which in turn is responsible for increased expression of GAP1. Experiments using an artificial UAS (upstream activating site) consisting of three GATAAGATAAG sites revealed that Nil2p exerts its effect by competing primarily with Nil1p and less effectively with Gln3p for these sites. Apparently, the principal role of Nil2p is to prevent activation of transcription by Nil1p unless Nil1p has been converted to a more active state by the absence of glutamine and glutamate.

References

Jul 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·Y H Fu, G A Marzluf
Feb 1, 1988·Journal of Bacteriology·W E Courchesne, B Magasanik
Jul 17, 1973·Biochemical and Biophysical Research Communications·R DrillienF Lacroute
Oct 10, 1995·Proceedings of the National Academy of Sciences of the United States of America·M StanbroughB Magasanik

❮ Previous
Next ❯

Citations

Jan 21, 2000·FEMS Microbiology Reviews·E G ter SchureC T Verrips
Mar 16, 2012·Genetics·Per O Ljungdahl, Bertrand Daignan-Fornier
Mar 12, 2016·PLoS Computational Biology·Andreas Milias-ArgeitisJohn Lygeros
Aug 7, 1999·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·M WonH S Yoo
Dec 11, 2013·The Journal of Biological Chemistry·Rajendra RaiTerrance G Cooper
Feb 27, 1999·Molecular Microbiology·S Soussi-Boudekou, B André
Apr 17, 1999·Molecular and Cellular Biology·I IraquiA Urrestarazu
Feb 20, 2007·Molecular and Cellular Biology·Patrice GodardBruno André
Jun 14, 2002·Gene·Boris Magasanik, Chris A Kaiser
Oct 24, 2007·Fungal Genetics and Biology : FG & B·Wei-Li LiaoWilliam A Fonzi

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.