Role of GerKB in germination and outgrowth of Clostridium perfringens spores

Applied and Environmental Microbiology
D Paredes-SabjaM R Sarker

Abstract

Previous work indicated that Clostridium perfringens gerKA gerKC spores germinate significantly, suggesting that gerKB also has a role in C. perfringens spore germination. We now find that (i) gerKB was expressed only during sporulation, likely in the forespore; (ii) gerKB spores germinated like wild-type spores with nonnutrient germinants and with high concentrations of nutrients but more slowly with low nutrient concentrations; and (iii) gerKB spores had lower colony-forming efficiency and slower outgrowth than wild-type spores. These results suggest that GerKB plays an auxiliary role in spore germination under some conditions and is required for normal spore viability and outgrowth.

References

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Dec 10, 2003·Current Opinion in Microbiology·P Setlow
Dec 18, 2007·Journal of Bacteriology·D Paredes-SabjaM R Sarker
Jan 29, 2008·International Journal of Food Microbiology·D Paredes-SabjaM R Sarker
May 13, 2008·Journal of Bacteriology·D Paredes-SabjaM R Sarker

Citations

Apr 2, 2013·Trends in Microbiology·Kai ReinekeDietrich Knorr
Apr 26, 2002·Journal of Applied Microbiology·M L CaipoD W Schaffner
May 16, 2002·Journal of Applied Microbiology·E MellyP Setlow
Jul 17, 2010·Future Microbiology·Michael MallozziGayatri Vedantam
May 2, 2013·Critical Reviews in Microbiology·M R SarkerD Paredes-Sabja
Nov 14, 2015·Expert Opinion on Therapeutic Targets·Can M Ünal, Michael Steinert
Feb 15, 2011·Food Microbiology·Yinghua XiaoMarjon H J Wells-Bennik
Aug 19, 2014·Research in Microbiology·Valeria Olguín-AranedaD Paredes-Sabja
Jul 20, 2016·Journal of Bacteriology·Disha BhattacharjeeJoseph A Sorg

Related Concepts

Bacterial Proteins
Clostridium perfringens
Spores, Bacterial
Gene Deletion
MRNA Differential Display
Gene Order
Virus Viability
Cell Survival
Spores, Fungal
Germine

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