Role of GerKB in germination and outgrowth of Clostridium perfringens spores

Applied and Environmental Microbiology
D Paredes-SabjaM R Sarker


Previous work indicated that Clostridium perfringens gerKA gerKC spores germinate significantly, suggesting that gerKB also has a role in C. perfringens spore germination. We now find that (i) gerKB was expressed only during sporulation, likely in the forespore; (ii) gerKB spores germinated like wild-type spores with nonnutrient germinants and with high concentrations of nutrients but more slowly with low nutrient concentrations; and (iii) gerKB spores had lower colony-forming efficiency and slower outgrowth than wild-type spores. These results suggest that GerKB plays an auxiliary role in spore germination under some conditions and is required for normal spore viability and outgrowth.


Jan 1, 1990·Annual Review of Microbiology·A Moir, D A Smith
Jan 1, 1994·Society for Applied Bacteriology Symposium Series·A MoirB M Corfe
Apr 20, 2002·Cellular and Molecular Life Sciences : CMLS·A MoirJ Behravan
Apr 3, 2003·Journal of Bacteriology·Rosa-Martha Cabrera-MartinezP Setlow
Aug 13, 2003·Journal of Applied Microbiology·Barbara SetlowP Setlow
Dec 10, 2003·Current Opinion in Microbiology·P Setlow
Dec 18, 2007·Journal of Bacteriology·D Paredes-SabjaM R Sarker
Jan 29, 2008·International Journal of Food Microbiology·D Paredes-SabjaM R Sarker
May 13, 2008·Journal of Bacteriology·D Paredes-SabjaM R Sarker


Apr 2, 2013·Trends in Microbiology·Kai ReinekeDietrich Knorr
Apr 26, 2002·Journal of Applied Microbiology·M L CaipoD W Schaffner
May 16, 2002·Journal of Applied Microbiology·E MellyP Setlow
Jul 17, 2010·Future Microbiology·Michael MallozziGayatri Vedantam
May 2, 2013·Critical Reviews in Microbiology·M R SarkerD Paredes-Sabja
Nov 14, 2015·Expert Opinion on Therapeutic Targets·Can M Ünal, Michael Steinert
Feb 15, 2011·Food Microbiology·Yinghua XiaoMarjon H J Wells-Bennik
Aug 19, 2014·Research in Microbiology·Valeria Olguín-AranedaD Paredes-Sabja
Jul 20, 2016·Journal of Bacteriology·Disha BhattacharjeeJoseph A Sorg

Related Concepts

Bacterial Proteins
Clostridium perfringens
Spores, Bacterial
Gene Deletion
MRNA Differential Display
Gene Order
Virus Viability
Cell Survival
Spores, Fungal

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