Role of glutathione and catalase in H2O2 detoxification in LPS-activated hepatic endothelial and Kupffer cells

The American Journal of Physiology
Z Spolarics, J X Wu

Abstract

The present study investigated the effect of lipopolysaccharide (LPS; from Escherichia coli, 2 mg/kg body wt ip) on selected aspects of the antioxidant status in Kupffer and sinusoidal endothelial cells. Cells were isolated 18 h after the injection of saline or LPS. In fresh suspension cultures, cellular reduced glutathione (GSH) and H2O2 were determined by monochlorobimane, and 2',7'-dichlorofluorescein diacetate, respectively, using a fluorescence plate reader. LPS injection increased GSH content two- to threefold in Kupffer cells compared with cells from control rats. Cellular GSH content was higher in endothelial than Kupffer cells. However, LPS did not increase GSH content in endothelial cells. Addition of H2O2 (40-200 microM) to Kupffer or endothelial cells caused a transient decrease in GSH, which was more pronounced in cells from control rats (approximately 45% drop) than in LPS-exposed cells (approximately 25% drop). Depleted GSH levels were accompanied by a proportional increase in cellular H2O2. After inhibition of catalase by 3-amino-1,2,4-triazole, the presence of 0.2 mM H2O2 depleted GSH content by 75% and 40% in Kupffer cells from saline- or LPS-injected rats, respectively. The same treatments caused a similar 50...Continue Reading

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Citations

Mar 28, 2012·Digestive Diseases and Sciences·Renata Minuzzo HartmannNorma Possa Marroni
Feb 17, 2017·Physiology·Laura BierhanslPeter Carmeliet
Jun 3, 2021·Journal of Biochemical and Molecular Toxicology·S Shehna MahimC Aruna Devi

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