Role of glutathione, ROS, and Bcl-xL in the inhibition of apoptosis of monocyte-derived dendritic cells by Leishmania mexicana promastigotes

Parasitology Research
Jorge Rodríguez-GonzálezLaila Gutiérrez-Kobeh

Abstract

Dendritic cells (DCs) are one of the principal host cells of the obligate intracellular parasite Leishmania that can survive and reproduce within cells due to the ability to regulate different cellular events, including apoptosis. Inhibition of host cell apoptosis is a strategy employed by multiple pathogens to ensure their survival in the infected cell. We have previously reported that Leishmania mexicana promastigotes and amastigotes inhibit camptothecin-induced apoptosis of monocyte-derived dendritic cells (moDCs) through the downregulation of p38 and JNK phosphorylation. The upregulation of glutathione (GSH), the most important regulator of reactive oxygen species (ROS) concentration, has proven to protect cells from apoptosis through the inhibition of JNK1. Another mechanism employed by cells for the protection of apoptosis is the expression of anti-apoptotic proteins of the Bcl-2 family. The aim of this study was to determine if GSH, ROS, and Bcl-xL participate in the inhibition of camptothecin-induced apoptosis of moDC by L. mexicana promastigotes. GSH quantification assays showed that camptothecin and BSO (an inhibitor of glutathione synthesis) strongly decreased intracellular GSH concentration in moDC, while infection ...Continue Reading

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Citations

Oct 6, 2020·Frontiers in Molecular Biosciences·Fangmei FuShiwu Zhang
Apr 4, 2021·International Journal of Molecular Sciences·Hamza A AlaswadNicola Jordan-Mahy

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Datasets Mentioned

BETA
GM-CSF

Methods Mentioned

BETA
density gradient centrifugation
Protein Assay
X-ray
Assay
Fluorescence
transfection

Software Mentioned

Quantity One
GraphPad Prism
GraphPad

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