Role of group VIA calcium-independent phospholipase A2 in arachidonic acid release, phospholipid fatty acid incorporation, and apoptosis in U937 cells responding to hydrogen peroxide

The Journal of Biological Chemistry
Rebeca PérezJesús Balsinde

Abstract

Group VIA calcium-independent phospholipase A2 (iPLA2) has been shown to play a major role in regulating basal phospholipid deacylation reactions in certain cell types. More recently, roles for this enzyme have also been suggested in the destruction of membrane phospholipid during apoptosis and after oxidant injury. Proposed iPLA2 roles have rested heavily on the use of bromoenol lactone as an iPLA2-specific inhibitor, but this compound actually inhibits other enzymes and lipid pathways unrelated to PLA2, which makes it difficult to define the contribution of iPLA2 to specific functions. In previous work, we pioneered the use of antisense technology to decrease cellular iPLA2 activity as an alternative approach to study iPLA2 functions. In the present study, we followed the opposite strategy and prepared U937 cells that exhibited enhanced iPLA activity by stably expressing a plasmid containing iPLA2 cDNA. Compared with control cells, the iPLA2 -overexpressing U937 cells showed elevated responses to hydrogen peroxide with regard to both arachidonic acid mobilization and incorporation of the fatty acid into phospholipids, thus providing additional evidence for the key role that iPLA2 plays in these events. Long-term exposure of t...Continue Reading

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Citations

Aug 20, 2011·The Journal of Biological Chemistry·Alma M AstudilloJesús Balsinde
Dec 22, 2012·The Journal of Biological Chemistry·Hanan ElimamAndrey V Cybulsky
May 27, 2006·The Journal of Biological Chemistry·Konstantin SeleznevZhongmin Alex Ma
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Dec 3, 2014·Biochimica Et Biophysica Acta·Carlos GuijasJesús Balsinde
Aug 4, 2011·Particle and Fibre Toxicology·Susanne Fritsch-DeckerSilvia Diabaté
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Jun 27, 2006·Journal of Lipid Research·Paul A WilsonDaniel J Crowther
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Mar 7, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Violeta RuipérezJesús Balsinde
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Sep 13, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Andreas NievergeltJürg Gertsch
Mar 14, 2008·The FEBS Journal·María A BalboaJesús Balsinde

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