Role of growth factors on extracellular matrix production by chick embryo fibroblasts in vitro. Antagonist effect of TGF-beta through the control of IL-1 and IL-1Ra secretion

Cytokine
M BodoE Becchetti

Abstract

Growth factors and extracellular matrix (ECM) macromolecules create specific environments that regulate cell proliferation and differentiation during embryonic development. This study had two main aims: (1) to characterize the phenotype of 7- and 13-day-old chick embryo fibroblasts treated with interleukin 1 (IL-1) and interleukin 6 (IL-6) evaluating in particular the neosynthesis of the total proteins and of the fibronectin (FN); (2) to examine the mechanisms through which transforming growth factor beta (TGF-beta) antagonizes the action of IL-1. The results show that protein neosynthesis and secretion is modulated by treatment with IL-1 and IL-6 only in 7-day-old fibroblasts. IL-1 favours cellular accumulation, while IL-6 promotes secretion more. FN, isolated by affinity chromatography and analysed by SDS-PAGE and flurography, is produced in greater concentrations by 7-day-old fibroblasts. Treatment with IL-1 promotes the accumulation of FN in the cellular compartment both at 7 and 13 days, while it stimulates the secretion only in 7-day-old fibroblasts. IL-6 only has a stimulating effect on the processes of accumulation and secretion on 13-day-old fibroblasts. The authors show in addition, that TFG-beta blocks the IL-1 induc...Continue Reading

Citations

Jun 17, 1998·European Journal of Cell Biology·M BodoPaolo Carinci
Mar 18, 2004·Radiographics : a Review Publication of the Radiological Society of North America, Inc·Ronald B J GlassThomas P Naidich
Jun 10, 2005·The FEBS Journal·Farid RahimiCarolyn L Geczy
Apr 6, 2001·Journal of Investigative Medicine : the Official Publication of the American Federation for Clinical Research·Tiziano BaroniG Abbritti
Jul 14, 2006·International Journal of Pediatric Otorhinolaryngology·Rosario RulloFrancesco Carinci
Nov 15, 2012·Biomaterials·Mark D SwartzlanderStephanie J Bryant
Mar 30, 2007·Osteoarthritis and Cartilage·E N Blaney DavidsonW B van den Berg
Jun 20, 2012·The Journal of Physiology·Ye Chun RuanSylvie Breton
Jul 14, 1998·The Journal of Clinical Endocrinology and Metabolism·E A KollerS N Malozowski
Jul 6, 2000·Journal of Biomedical Materials Research·Paola LocciE Becchetti
Apr 12, 2002·International Journal of Cancer. Journal International Du Cancer·Cinzia LilliPaola Locci

Related Concepts

Chick Embryo
Zyderm
Extracellular Matrix
Fibroblasts
Fibronectins
Glycosaminoglycans
Interleukin-1
Protein Biosynthesis
Interleukin-6
Transforming Growth Factor beta

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