PMID: 11336789May 5, 2001Paper

Role of guanine nucleotides in the regulation of the Ras/cAMP pathway in Saccharomyces cerevisiae

Biochimica Et Biophysica Acta
S RudoniEnzo Martegani

Abstract

The CDC25 gene product is a guanine nucleotide exchange factor for Ras proteins in yeast. Recently it has been suggested that the intracellular levels of guanine nucleotides may influence the exchange reaction. To test this hypothesis we measured the levels of nucleotides in yeast cells under different growth conditions and the relative amount of Ras2-GTP. The intracellular GTP/GDP ratio was found to be very sensitive to growth conditions: the ratio is high, close to that of ATP/ADP during exponential growth, but it decreases rapidly before the beginning of stationary phase, and it drops further under starvation conditions. The addition of glucose to glucose-starved cells causes a fast increase of the GTP/GDP ratio. The relative amount of Ras2-GTP changes in a parallel way suggesting that there is a correlation with the cytosolic GTP/GDP ratio. In addition 'in vitro' mixed-nucleotide exchange experiments done on purified Ras2 protein demonstrated that the GTP and GDP concentrations influence the extent of Ras2-GTP loading giving further support to their possible regulatory role.

References

Oct 15, 1984·European Journal of Biochemistry·E MarteganiM Baroni
Dec 1, 1994·Yeast·J M Thevelein
Dec 16, 1993·Nature·M S Boguski, F McCormick
Dec 1, 1996·Current Biology : CB·S J Taylor, D Shalloway
Aug 5, 1998·Nature·P A Boriack-SjodinJ Kuriyan

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Citations

Nov 30, 2002·Current Opinion in Microbiology·Paul K Herman
Feb 18, 2010·Molecular and Cellular Biology·Vernon T PhanFrank McCormick
Jul 24, 2012·EURASIP Journal on Bioinformatics & Systems Biology·Daniela BesozziEnzo Martegani
Nov 19, 2013·PloS One·Serena BroggiSonia Colombo
Mar 20, 2014·Cell Biochemistry and Biophysics·Azadeh Meshkini
Oct 4, 2013·Oxidative Medicine and Cellular Longevity·Loredana AmigoniSonia Colombo
Nov 22, 2005·The International Journal of Biochemistry & Cell Biology·Francesca MagheriniMarco Vanoni
Nov 7, 2012·The International Journal of Biochemistry & Cell Biology·Serena BroggiSonia Colombo
May 12, 2012·Biochimica Et Biophysica Acta·Fiorella BelottiEnzo Martegani
Mar 15, 2011·Toxicology and Applied Pharmacology·Azadeh MeshkiniKazem Nouri
May 26, 2017·ELife·Andrew Weems, Michael McMurray
May 18, 2016·Genetics·Thomas E DeverGraham D Pavitt
Apr 26, 2011·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Kyung-Chul WooKyong-Tai Kim
Aug 23, 2002·The Journal of Biological Chemistry·Kuo-Kuang WenPeter A Rubenstein
Sep 2, 2004·The Journal of Biological Chemistry·Sonia ColomboEnzo Martegani
Aug 10, 2017·Biomolecules·Raffaele NicastroClaudio De Virgilio
Aug 2, 2001·Yeast
Jul 29, 2021·Cell Reports·Syed S HussainDavid F Kashatus

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