Role of guanosine tetraphosphate in gene expression and the survival of glucose or seryl-tRNA starved cells of Escherichia coli K12

Molecular & General Genetics : MGG
T Nystöm

Abstract

The concentration of guanosine 3',5'-bispyrophosphate (ppGpp) increases in bacteria in response to amino acid or carbon/energy source starvation. An Escherichia coli K12 delta relA delta spoT mutant lacking the ability to synthesize ppGpp lost viability at an increased rate during both glucose and seryl-tRNA starvation. Also, the deleterious effect of chloramphenicol on starved wild-type cells could be overcome by inducing expression of RelA from a plasmid carrying the relA gene transcribed from a tac promoter, prior to starvation and chloramphenicol treatment. As demonstrated by two dimensional gel electrophoresis, this induction of the RelA protein resulted in global alterations in gene expression including increased synthesis of some rpoS-dependent proteins. The delta relA delta spoT mutant maintained high expression of several ribosomal proteins during starvation and appeared to exhibit significantly decreased translational fidelity, as demonstrated by an unusual heterogeneity in the isoelectric point of several proteins and the failure to express higher molecular weight proteins during starvation. Moreover, both rpoS-dependent and independent genes failed to exhibit increased expression in the mutant. It is suggested that ...Continue Reading

References

Jan 1, 1979·Annual Review of Genetics·J A Gallant
Oct 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·C Li, S Clarke
Dec 1, 1990·Journal of Bacteriology·T NyströmS Kjelleberg
Dec 1, 1990·Electrophoresis·R A VanBogelenF C Neidhardt
Jan 1, 1991·Journal of Bacteriology·I von OssowskiP C Loewen
Aug 1, 1990·Journal of Bacteriology·A TormoR Kolter
Aug 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·R A VanBogelen, F C Neidhardt
Jul 24, 1972·European Journal of Biochemistry·E Lund, N O Kjeldgaard
Sep 1, 1974·Journal of Bacteriology·F C NeidhardtD F Smith
Jun 1, 1971·Journal of Bacteriology·T Tosa, L I Pizer

❮ Previous
Next ❯

Citations

Feb 20, 1997·Biotechnology and Bioengineering·N DedhiaW Chen
Aug 1, 1997·Electrophoresis·J OstlingS Kjelleberg
May 14, 1999·Current Opinion in Microbiology·T Nyström
May 3, 2005·Nature Reviews. Microbiology·Kenn GerdesAnders Løbner-Olesen
Oct 10, 1998·Molecular Microbiology·A FarewellT Nyström
May 23, 2002·Genes & Development·Miki JishageThomas Nyström
May 20, 2004·Journal of Bacteriology·Margaret BrennerMitchell Singer
Jun 9, 2006·Microbiology and Molecular Biology Reviews : MMBR·Mee-Jung Han, Sang Yup Lee
Aug 20, 2014·FEMS Microbiology Reviews·Agata L StarostaDaniel N Wilson
Oct 19, 2004·Annual Review of Microbiology·Thomas Nyström
Jan 7, 2004·Biotechnology and Bioengineering·Pat S Lee, Kelvin H Lee
Sep 5, 2001·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·K IzutsuC Wada
Nov 9, 2000·Letters in Applied Microbiology·A M CrosseR R England
Feb 5, 2014·Antimicrobial Agents and Chemotherapy·Dariusz NowickiGrzegorz Węgrzyn
Nov 8, 2002·The Journal of Biological Chemistry·Lisa U MagnussonAnne Farewell
Feb 27, 1999·Molecular Microbiology·P DuwatA Gruss
Feb 24, 1998·Journal of Bacteriology·D RockabrandP Blum
Aug 7, 1998·Biochimica Et Biophysica Acta·T Nyström, N Gustavsson
Dec 1, 1995·Journal of Bacteriology·J OstlingS Kjelleberg

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.