Role of Gut Inflammation in Altering the Monocyte Compartment and Its Osteoclastogenic Potential in HLA-B27-Transgenic Rats

Arthritis & Rheumatology
Cecilia AnsaloneCarl S Goodyear

Abstract

To investigate the relationship between intestinal inflammation and the central and peripheral innate immune system in the pathogenesis of HLA-B27-associated spondyloarthritis using an HLA-B27-transgenic (B27-Tg) rat model. The myeloid compartment of the blood and bone marrow (BM) of B27-Tg rats, as well as HLA-B7-Tg and non-Tg rats as controls, was evaluated by flow cytometry. Plasma from rats was assessed by enzyme-linked immunosorbent assay for levels of CCL2 and interleukin-1α (IL-1α). Rats were treated with antibiotics for 4 weeks, and the myeloid compartment of the blood and BM was evaluated by flow cytometry. The osteoclastogenic potential of BM-derived cells from antibiotic-treated rats, in the presence or absence of tumor necrosis factor (TNF), was evaluated in vitro. B27-Tg rats had substantially higher numbers of circulating Lin-CD172a+CD43low monocytes as compared to control animals, and this was significantly correlated with higher levels of plasma CCL2. Antibiotic treatment of B27-Tg rats markedly reduced the severity of ileitis, plasma levels of CCL2 and IL-1α, and number of BM and blood Lin-CD172a+CD43low monocytes, a cell subset shown in the present study to have the greatest in vitro osteoclastogenic potential...Continue Reading

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Citations

May 17, 2019·Nature Reviews. Rheumatology·Robert BuschElizabeth D Mellins
Jun 2, 2017·Nature Reviews. Rheumatology·Joanna Collison
Nov 28, 2019·Journal of Immunology Research·Huihui XuHongyan Zhao
Dec 19, 2019·International Journal of Molecular Sciences·Ke KeYousef Abu-Amer
Mar 20, 2019·Frontiers in Cellular and Infection Microbiology·Lianjun YangHai Lu
Feb 2, 2021·Current Osteoporosis Reports·Julie Kristine KnudsenSuzette Sørensen
Oct 24, 2020·Biomolecules·Aigul Sharip, Jeannette Kunz
Nov 27, 2020·Therapeutic Advances in Musculoskeletal Disease·Gavin Clunie, Nicole Horwood
Feb 25, 2021·Seminars in Immunopathology·Zoya QaiyumRobert D Inman
Mar 17, 2020·Best Practice & Research. Clinical Rheumatology·Maxime BrebanSimon Glatigny

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