Role of heme-hemopexin in human T-lymphocyte proliferation

Experimental Cell Research
A SmithR C Hunt

Abstract

Heme-hemopexin supports and stimulates proliferation of human acute T-lymphoblastic (MOLT-3) cells, suggesting the participation of heme in cell growth and division. MOLT-3 cells express approximately 58,000 hemopexin receptors per cell (apparent Kd 20 nM), of which about 20% are on the cell surface. Binding is dose- and temperature-dependent, and growth in serum-free IMDM medium is stimulated by 100-1000 nM heme-hemopexin, consistent with the high affinity of the receptor for hemopexin, and maximal growth is seen in response to 500 nM complex. Growth was similar in defined minimal medium supplemented with either low concentrations of heme-hemopexin or iron-transferrin, and either of these complexes were about 80% as effective as a serum supplement. Heme-hemopexin, but not apo-hemopexin, reversed the growth inhibition caused by desferrioxamine showing that heme-iron derived from heme catabolism is used for cell growth. Cobalt-protoporphyrin (CoPP)-hemopexin, which binds to the receptor but is not transported intracellularly [Smith et al., (1993) J. Biol. Chem. 268, 7365], also stimulated cell proliferation in serum-free IMDM but did not "rescue" the cells from desferrioxamine. Furthermore, CoPP-hemopexin effectively competed fo...Continue Reading

References

Jan 1, 1979·Journal of Supramolecular Structure·J W Larrick, P Cresswell
Dec 28, 1979·Biochemical and Biophysical Research Communications·D M DaviesW T Morgan
Aug 1, 1991·American Journal of Clinical Pathology·R L HamillB A Cook
Jan 15, 1991·Biochemical and Biophysical Research Communications·H StepienM Pawlikowski
Mar 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·N BerryY Nishizuka
Feb 1, 1989·Cell Structure and Function·H OkazakiY Kobayashi
Jan 1, 1988·Annual Review of Biochemistry·P Reichard
Jan 1, 1987·Methods in Enzymology·G Di SabatoL Thompson
Mar 15, 1974·European Journal of Biochemistry·Z HrkalI Kalousek
May 1, 1995·The Biochemical Journal·H P van DijkU Muller-Eberhard
Jul 1, 1996·Journal of Cellular Physiology·R C HuntA Smith

❮ Previous
Next ❯

Citations

Jan 11, 2000·The Proceedings of the Nutrition Society·J H Brock, V Mulero
Jul 17, 2009·Journal of Proteome Research·Jennifer E GrantKevin L Schey
Jun 4, 2002·DNA and Cell Biology·Emanuela Tolosano, Fiorella Altruda
Aug 5, 2009·Antioxidants & Redox Signaling·Emanuela TolosanoVeronica Fiorito
Nov 9, 2001·Cell Structure and Function·K SuzukiH Namiki
Nov 29, 2008·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Ann SmithRachel M Helston
Aug 17, 2004·American Journal of Physiology. Gastrointestinal and Liver Physiology·Aliye UcBradley E Britigan
Jan 4, 1998·Journal of Cellular Biochemistry·B M DeramaudtN G Abraham
Dec 8, 2010·Proteomics. Clinical Applications·Baharak Vafadar-IsfahaniBalwir Matharoo-Ball
Aug 23, 2007·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Meghan M FlahertyAlvin L Crumbliss
Mar 22, 2003·Blood·Frank A D T G WagenerCarl G Figdor
Oct 3, 2001·Clinica Chimica Acta; International Journal of Clinical Chemistry·J R Delanghe, M R Langlois
May 24, 2003·Journal of Hepatology·Yasuyuki FukuharaGozoh Tsujimoto

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.