Role of herpes simplex virus ICP27 in the degradation of mRNA by virion host shutoff RNase.

Journal of Virology
Brunella TaddeoBernard Roizman

Abstract

The virion host shutoff (VHS) RNase tegument protein released into cells by infecting virus has two effects. Preexisting stable mRNAs (e.g., GAPDH [glyceraldehyde-3-phosphate dehydrogenase]) are rapidly degraded. Stress response RNAs containing AU-rich elements (AREs) in the 3' untranslated region (3'UTR) are deadenylated and cleaved, but the cleavage products persist for hours, in contrast to the short half-lives of ARE-containing mRNAs in uninfected cells. At late times, the VHS RNase is neutralized by the viral structural proteins VP16 and VP22. A recent study (J. A. Corcoran, W. L. Hsu, and J. R. Smiley, J. Virol. 80:9720-9729, 2006) reported that, at relatively late times after infection, ARE RNAs are rapidly degraded in cells infected with DeltaICP27 mutant virus and concluded that ICP27 "stabilizes" ARE mRNAs. We report the following. (i) The rates of degradation of ARE mRNA at early times (3 h) after infection with the wild type or the DeltaICP27 mutant virus are virtually identical, and hence ICP27 plays no role in this process. (ii) In noncomplementing cells, VHS RNase or VP22 is not synthesized. Therefore, the only VHS that is active is brought into cells by the DeltaICP27 mutant. (ii) The VHS RNase brought into the ...Continue Reading

References

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Jul 9, 2009·Proceedings of the National Academy of Sciences of the United States of America·Brunella TaddeoBernard Roizman

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Citations

Apr 17, 2013·Proceedings of the National Academy of Sciences of the United States of America·Minfeng ShuBernard Roizman
Aug 1, 2013·Wiley Interdisciplinary Reviews. RNA·G Sullivan Read
May 21, 2015·Nature Communications·Andrzej J RutkowskiLars Dölken
Apr 9, 2020·PLoS Pathogens·Raecliffe DalyMarta Maria Gaglia
Aug 19, 2021·The Journal of General Virology·Yeu-Yang TsengDavid C Tscharke

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