Role of high glucose-induced nuclear factor-kappaB activation in monocyte chemoattractant protein-1 expression by mesangial cells.

Journal of the American Society of Nephrology : JASN
Hunjoo HaHi Bahl Lee

Abstract

Although high glucose (HG) has been shown to induce nuclear factor-kappaB (NF-kappaB) activation in vascular cells, the upstream regulation and the biologic significance of NF-kappaB activation in diabetic renal injury are not clear. It was, therefore, examined if HG-induced generation of reactive oxygen species (ROS) and protein kinase C (PKC) activation are involved in NF-kappaB activation in mesangial cells (MC), and the role of NF-kappaB activation in HG-induced monocyte chemoattractant protein-1 (MCP-1) expression by MC was further investigated. Recent observations suggest that MCP-1 may play a role in the development and progression of diabetic nephropathy. HG rapidly induced NF-kappaB activation in MC as estimated by electrophoretic mobility shift assay. Supershift assay suggests that most of the binding activity arose from p50/p50 and p50/p65 dimers. Antioxidants, pyrrolidine dithiocarbamate, N-acetyl-L-cystein, and trolox effectively inhibited HG-induced NF-kappaB activation in MC. HG rapidly generated dichlorofluorescin-sensitive intracellular ROS in MC as measured by laser-scanning confocal microscopy. HG also activated PKC rapidly in MC. Inhibition of PKC effectively blocked HG-induced intracellular ROS generation a...Continue Reading

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Citations

Mar 9, 2021·Future Medicinal Chemistry·Nimeet DesaiRakesh K Tekade
Feb 22, 2003·Journal of the American Society of Nephrology : JASN·Geun Taek LeeYoung Dong Cho
Apr 23, 2003·Journal of the American Society of Nephrology : JASN·Masakazu HanedaRyuichi Kikkawa
Jul 23, 2003·Journal of the American Society of Nephrology : JASN·Hi Bahl LeeHunjoo Ha
Jul 23, 2003·Journal of the American Society of Nephrology : JASN·Hunjoo Ha, Hi Bahl Lee
May 2, 2021·Reviews in Endocrine & Metabolic Disorders·Hassane ZouhalAyoub Saeidi
Jun 5, 2020·European Journal of Nutrition·Simone H CrouchAletta E Schutte
Sep 19, 2003·Biochemical and Biophysical Research Communications·Zongpei JiangHi Bahl Lee
Aug 4, 2004·Proceedings of the National Academy of Sciences of the United States of America·Changyong LuHelen Vlassara
Jul 11, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Fevzi BedirHalis Suleyman
Dec 16, 2004·American Journal of Physiology. Renal Physiology·Ho Jae HanMary Taub
Apr 14, 2005·American Journal of Physiology. Renal Physiology·Judy B de HaanGregory H Tesch
Nov 3, 2005·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Leyi GuYasuhiko Tomino
Feb 13, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Ertugrul ErhanHalis Suleyman
Apr 29, 2005·Journal of the American Society of Nephrology : JASN·Florian Lang
Feb 10, 2006·American Journal of Physiology. Renal Physiology·Thangavel SamikkannuThomas J Thekkumkara
Jan 6, 2006·Journal of the American Society of Nephrology : JASN·Sarah X ZhangJian-Xing Ma
Feb 21, 2006·Journal of the American College of Cardiology·Juan Asbun, Francisco J Villarreal
Sep 20, 2005·The Journal of Nutritional Biochemistry·David A AllenSteven M Harwood
Dec 15, 2006·Journal of the American Society of Nephrology : JASN·Sudhir V ShahVivian A Fonseca
Dec 21, 2006·Archives of Physiology and Biochemistry·Elena Dragomir, Maya Simionescu
Dec 23, 2006·Antioxidants & Redox Signaling·Melinda T CoughlanJosephine M Forbes

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