Role of high-mobility group box-1 in myocardial ischemia/reperfusion injury and the effect of ethyl pyruvate

Experimental and Therapeutic Medicine
Yunling LinJun Fang

Abstract

High-mobility group box-1 (HMGB1) acts as a proinflammatory cytokine that triggers and amplifies the inflammation cascade following ischemia/reperfusion (I/R). Ethyl pyruvate (EP) has been reported to inhibit HMGB1 release in several I/R models. This study was designed to investigate the potential role of HMGB1 in a rat myocardial I/R model and to determine the effect of EP. Male Sprague Dawley rats were subjected to 30 min myocardial ischemia and 48 h reperfusion. In protocol 1, the rats were assigned to one of four groups (n=16 per group): Phosphate-buffered saline (PBS) or recombinant human HMGB1 (rhHMGB1) at three different doses (1, 10 or 100 μg/kg). In protocol 2, the rats were also assigned to one of four groups (n=16 per group): Sham, control, EP and EP + rhHMGB1. EP (40 mg/kg) or rhHMGB1 (100 μg/kg) was injected intravenously prior to reperfusion. Hemodynamic measurements were performed, and myocardial infarct size (IS) was calculated. Western blotting was conducted to evaluate HMGB1, tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) expression levels. In the protocol 1 rats, the IS was markedly increased in the rhHMGB1 (100 μg/kg) group compared with that in the PBS group, and this increase was accompanied by e...Continue Reading

References

Jun 18, 2002·American Journal of Physiology. Gastrointestinal and Liver Physiology·Runkuan YangMitchell P Fink
Jul 12, 2002·Nature·Paola ScaffidiMarco E Bianchi
Sep 5, 2002·Proceedings of the National Academy of Sciences of the United States of America·Luis UlloaKevin J Tracey
Apr 30, 2004·The Journal of Thoracic and Cardiovascular Surgery·Y Joseph WooTodd Grand
Mar 30, 2005·The Journal of Experimental Medicine·Allan TsungTimothy R Billiar
Sep 6, 2005·Stroke; a Journal of Cerebral Circulation·Young-Mi YuJa-Kyeong Lee
Feb 20, 2007·Cardiovascular Research·Maurits T DirksenDirk J G M Duncker
Sep 15, 2007·The New England Journal of Medicine·Derek M Yellon, Derek J Hausenloy
Apr 24, 2008·Molecular Medicine·John R KluneAllan Tsung
Jun 20, 2008·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Jialong GuoShunqing Zuo
Jun 25, 2008·Circulation·Martin AndrassyAngelika Bierhaus
Sep 11, 2008·Cardiovascular & Hematological Disorders Drug Targets·Ming Zhang, Li Chen
Jul 5, 2012·American Journal of Physiology. Renal Physiology·May M RabadiBrian B Ratliff
Apr 29, 2014·International Journal of Cardiology·Xiaorong HuHong Jiang

❮ Previous
Next ❯

Citations

Apr 2, 2015·International Journal of Cardiology·Zhaowei ZhuShenghua Zhou
Sep 1, 2015·Journal of Pharmacological Sciences·Li YaoXiaohua Wang
Jun 2, 2016·BMC Cardiovascular Disorders·Xing-Han TianMeng Chen
Oct 1, 2018·Journal of Cellular Biochemistry·Quan-Fang ChenXiang-Tao Zeng
Feb 21, 2019·Journal of Cellular and Molecular Medicine·Jiyang XueQifeng Zhao
Dec 17, 2016·Journal of Inflammation·Runkuan YangTor Inge Tonnessen
Oct 27, 2015·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Yan Fei QiMohan K Raizada
Apr 18, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Abdul WahidXiaohong Tang
Dec 12, 2018·Pharmacology & Therapeutics·Laura PellegriniFederica Limana
Jul 25, 2021·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Chenxi LuQiang Wang
Jun 2, 2017·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Baoqing ChenYou Lu
Oct 6, 2021·Mycopathologia·JiaoJiao WangHang Sun
Nov 20, 2016·Current Opinion in Organ Transplantation·Hsi-Min HsiaoDaniel Kreisel

❮ Previous
Next ❯

Methods Mentioned

BETA
protein assay
electrophoresis

Software Mentioned

SPSS

Related Concepts

Related Feeds

Cardiovascular Inflammation

Inflammation plays a significant role in the development of cardiovascular diseases, an understanding of these endogenous processes is critical for evaluating the risks and potential treatment strategies. Discover the latest research on cardiovascular inflammation here.

Cardiomyopathy

Cardiomyopathy is a disease of the heart muscle, that can lead to muscular or electrical dysfunction of the heart. It is often an irreversible disease that is associated with a poor prognosis. There are different causes and classifications of cardiomyopathies. Here are the latest discoveries pertaining to this disease.