Role of high-mobility group box 1 in patients with acute obstructive suppurative cholangitis-induced sepsis

Journal of Inflammation Research
Akanand SinghJianping Gong

Abstract

High-mobility group box 1 (HMGB1) is a proinflammatory cytokine that plays an active role during the pathogenesis of inflammatory processes. The primary aim of this study was to detect whether HMGB1 is involved in the pathogenesis of acute obstructive suppurative cholangitis (AOSC). We collected peripheral blood samples from 23 patients with AOSC and 23 healthy volunteers who served as normal controls. All participants were tested for HMGB1 mRNA level, HMGB1 protein, tumor necrosis factor alpha (TNF-alpha), and interleukin 10 (IL-10). HMGB1 mRNA levels were tested using real-time polymerase chain reaction. HMGB1 protein expression was measured using Western blot. TNF-alpha and IL-10 were tested using enzyme-linked immunosorbent assay. The expression of HMGB1 mRNA and HMGB1 protein was higher in the AOSC group than in the normal controls (P<0.01), and the levels gradually decreased to normal after treatment of the disease (P<0.01). The content of TNF-alpha and IL-10 in peripheral blood of patients with AOSC was significantly higher than that of normal controls (P<0.01) but decreased to normal levels after the necessary treatment (P<0.01). The levels of HMGB1 mRNA and HMGB1 protein were elevated in patients with AOSC, which may p...Continue Reading

Citations

Oct 2, 2015·Aging and Disease·Tatiana BarichelloFelipe Dal-Pizzol
Apr 22, 2017·World Journal of Gastroenterology : WJG·Zong-Ming ZhangYue Zhao
Oct 6, 2021·Mycopathologia·JiaoJiao WangHang Sun

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Methods Mentioned

BETA
density gradient centrifuge
electrophoresis
enzyme-linked immunosorbent assay
ELISA

Software Mentioned

SPSS19
Gene Tools

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