PMID: 8965644Mar 1, 1996Paper

Role of highly conserved pyrimidine-rich sequences in the 3' untranslated region of the GAP-43 mRNA in mRNA stability and RNA-protein interactions

Brain Research. Molecular Brain Research
D T KohnN Perrone-Bizzozero

Abstract

We have shown previously that the mRNA for the growth-associated protein GAP-43 is selectively stabilized during neuronal differentiation. In this study, we explored the role of its highly conserved 3' untranslated region (3'UTR) in mRNA stability and RNA-protein interactions. The 3'UTRs of the rat and chicken GAP-43 mRNAs show 78% sequence identity, which is equivalent to the conservation of their coding regions. In rat PC12 cells stably transfected with the full-length rat or chicken GAP-43 cDNAs, the transgene mRNAs decayed with same half-life of about 3 h. The GAP-43 3'UTR also caused the rabbit beta-globin mRNA to decay with a half-life of 4 h, indicating that the major determinants for GAP-43 mRNA stability are localized in its highly conserved 3'UTR. Three brain cytosolic RNA-binding proteins (molecular mass 40, 65 and 95 kDa) were found to interact with both the rat and chicken GAP-43 mRNAs. These RNA-protein interactions were specific and involved pyrimidine-rich sequences in the 3'UTR. Like the GAP-43 mRNA, the activity of these proteins was enriched in brain and increased during development. We propose that highly conserved pyrimidine-rich sequences in the 3'UTR of this mRNA regulate GAP-43 gene expression via intera...Continue Reading

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Citations

Oct 20, 1999·Journal of Molecular Neuroscience : MN·S AronovI Ginzburg
Oct 16, 2004·Journal of Molecular Evolution·Todd H Oakley, Daniel R Huber
Sep 25, 2004·Journal of Neurobiology·Catherine L SmithRichard W Burry
Aug 29, 2012·Journal of Neurochemistry·Mahmoud L SolimanThad A Rosenberger
Feb 4, 2003·Journal of Neurochemistry·Martine UittenbogaardAnne Chiaramello
Jan 13, 2006·Journal of Neurochemistry·Federico BolognaniNora I Perrone-Bizzozero
Sep 14, 2007·Journal of Neuroscience Research·Federico Bolognani, Nora I Perrone-Bizzozero
Apr 10, 2007·Biochemical and Biophysical Research Communications·Hsiao-Huei ChenAlexandre F R Stewart
Feb 1, 1997·Trends in Neurosciences·L I Benowitz, A Routtenberg
Jun 20, 1998·The Journal of Biological Chemistry·R Cañete-SolerW W Schlaepfer
Oct 6, 2020·Frontiers in Cell and Developmental Biology·Daayun ChungGabriela Caraveo
Jun 20, 2007·Neurochemical Research·Federico BolognaniNora I Perrone-Bizzozero
May 30, 2002·The Journal of Biological Chemistry·Andrea C Beckel-MitchenerNora I Perrone-Bizzozero
Mar 24, 2006·Journal of Cell Science·Antonia RattiVincenzo Silani

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