Role of Host Cell-Derived Amino Acids in Nutrition of Intracellular Salmonella enterica

Infection and Immunity
Jasmin PoppMichael Hensel

Abstract

The facultative intracellular pathogen Salmonella enterica resides in a specific membrane-bound compartment termed the Salmonella-containing vacuole (SCV). Despite being segregated from access to metabolites in the host cell cytosol, Salmonella is able to efficiently proliferate within the SCV. We set out to unravel the nutritional supply of Salmonella in the SCV with focus on amino acids. We studied the availability of amino acids by the generation of auxotrophic strains for alanine, asparagine, aspartate, glutamine, and proline in a macrophage cell line (RAW264.7) and an epithelial cell line (HeLa) and examined access to extracellular nutrients for nutrition. Auxotrophies for alanine, asparagine, or proline attenuated intracellular replication in HeLa cells, while aspartate, asparagine, or proline auxotrophies attenuated intracellular replication in RAW264.7 macrophages. The different patterns of intracellular attenuation of alanine- or aspartate-auxotrophic strains support distinct nutritional conditions in HeLa cells and RAW264.7 macrophages. Supplementation of medium with individual amino acids restored the intracellular replication of mutant strains auxotrophic for asparagine, proline, or glutamine. Similarly, a mutant st...Continue Reading

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Citations

Feb 8, 2018·Environmental Microbiology Reports·Arthur ThompsonKarsten Tedin
Jul 4, 2017·Cellular Microbiology·Dirk Bumann, Joep Schothorst
Nov 17, 2016·Infection and Immunity·Patrick A McLaughlinAdrianus W M van der Velden
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Apr 10, 2021·Frontiers in Cellular and Infection Microbiology·Jennifer RöderMichael Hensel
Jul 22, 2017·Microbiology Spectrum·M Graciela Pucciarelli, Francisco García-Del Portillo
Jul 1, 2021·Microbiology Spectrum·Jack R McLeodCorrella S Detweiler

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