Role of Hsp70 synthesis in the fate of the insulin-receptor complex after heat shock in cultured fetal hepatocytes

Journal of Cellular Biochemistry
J L ZachayusC Plas

Abstract

The influence of a mild heat shock on the fate of the insulin-receptor complex was studied in cultured fetal rat hepatocytes whose insulin glycogenic response is sensitive to heat [Zachayus and Plas (1995): J Cell Physiol 162:330-340]. After exposure from 15 min to 2 hr at 42.5 degrees C, the amount of (125)1-insulin associated with cells at 37 degrees C was progressively decreased (by 35% after 1 hr), while the release of (125)1-insulin degradation products into the medium was also inhibited (by 75%), more than expected from the decrease in insulin binding. Heat shock did not affect the insulin-induced internalization of cell surface insulin receptors but progressively suppressed the recycling at 37 degrees C of receptors previously internalized at 42.5 degrees C in the presence of insulin. When compared to the inhibitory effects of chloroquine on insulin degradation and insulin receptor recycling, which were immediate (within 15 min), those of heat shock developed within 1 hr of heating. The protein level of insulin receptors was not modified after heat shock and during recovery at 37 degrees C, while that of Hsp72/73 exhibited a transitory accumulation inversely correlated with variations in insulin binding, as assayed by We...Continue Reading

References

Jun 1, 1992·The Journal of Cell Biology·R P BeckmannW J Welch
Nov 1, 1991·Experimental Cell Research·R L AndersonG M Hahn
Jan 1, 1990·Methods in Enzymology·G L Firestone, S D Winguth
Feb 1, 1986·Biochemical Society Transactions·W H Evans
Aug 1, 1988·Endocrine Reviews·W C Duckworth
Dec 15, 1983·European Journal of Biochemistry·C de Duve
Mar 15, 1983·Biochimica Et Biophysica Acta·S K Calderwood, G M Hahn
Dec 1, 1993·Cell Structure and Function·T Marunouchi, H Hosoya
Mar 1, 1994·Proceedings of the National Academy of Sciences of the United States of America·R P CiavarraF J Castora
Jun 1, 1994·The Journal of Cell Biology·P A WaltonW J Welch
Dec 1, 1964·Experimental Cell Research·V J EVANSE L SCHILLING

❮ Previous
Next ❯

Citations

Apr 25, 2018·Temperature : Multidisciplinary Biomedical Journal·Brett R ElyChristopher T Minson
May 30, 2006·American Journal of Physiology. Heart and Circulatory Physiology·Gefeng LiR William Currie
Mar 30, 1999·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·M U SiddiquiC Plas
Aug 23, 2008·Mechanisms of Development·Laura CobrerosMaría D Martín-Bermudo

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antimalarial Agents

Antimalarial agents, also known as antimalarials, are designed to prevent or cure malaria. Discover the latest research on antimalarial agents here.

Antimalarial Agents (ASM)

Antimalarial agents, also known as antimalarials, are designed to prevent or cure malaria. Discover the latest research on antimalarial agents here.