Role of human sulfide: quinone oxidoreductase in H2S metabolism

Methods in Enzymology
Michael R JacksonMarilyn Schuman Jorns

Abstract

The first step in the mammalian metabolism of H2S is catalyzed by sulfide:quinone oxidoreductase (SQOR). Human SQOR is an integral membrane protein, which presumably interacts with the inner mitochondrial membrane in a monotopic fashion. The enzyme is a member of a family of flavoprotein disulfide oxidoreductases (e.g., glutathione reductase) that utilize a Cys-S-S-Cys disulfide bridge as an additional redox center. SQOR catalyzes a two-electron oxidation of H2S to sulfane sulfur using coenzyme Q as electron acceptor. The enzyme also requires a third substrate to act as the acceptor of the sulfane sulfur from a cysteine persulfide intermediate. Here, we describe a method for the bacterial expression of human SQOR as a catalytically active membrane-bound protein, procedures for solubilization and purification of the recombinant protein to >95% homogeneity, and spectrophotometric assays to monitor SQOR-mediated H2S oxidation in reactions with different sulfane sulfur acceptors.

Citations

Feb 28, 2017·Medicinal Research Reviews·Yueqin ZhengBinghe Wang
Sep 11, 2019·Journal of Molecular Modeling·Jenner Bonanata, E Laura Coitiño
Jul 28, 2018·Oxidative Medicine and Cellular Longevity·Alessandro Giuffrè, João B Vicente
May 27, 2021·Nature Communications·Eizo MarutaniFumito Ichinose

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