PMID: 2483173Jan 1, 1988Paper

Role of hyperpolarization generated by the Na+ - K+ pump in the trans-synaptic induction of RNA synthesis in sympathetic neurons.

Journal of Physiology, Paris
V Gisiger

Abstract

1. The mechanism which enables activation of the nicotinic receptors to modify the synthesis of RNA was investigated in the incubated superior cervical ganglion of the rat. RNA labelling with [5-3H] uridine was used in order to screen the effects of varying the ionic environment and altering Na+ channels on the sequence of the three changes in ganglionic RNA synthesis induced by stimulation, i.e. a) an initial decrease, b) an increase during the late stages of stimulation, c) another increase taking place after the end of stimulation. These successive variations were obtained by either repetitive excitation of the preganglionic nerve, or application of ACh or carbachol to the ganglion. 2. The three changes in RNA synthesis mediated by ACh or carbachol were prevented when the KCl concentration of the medium was increased up to 37 mM or when NaCl of the medium was replaced with Tris or sucrose. This confirmed previous indications that the sequence of activity-induced changes is initiated by the transmembrane ionic fluxes mediated by nicotinic activation and not by depolarization per se. 3. Application of aconitine to resting ganglia for 1 h decreased the RNA synthesis to the same extent as a 1 h preganglionic or ACh stimulation. ...Continue Reading

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