Role of hypoxanthine and guanine in regulation of Salmonella typhimurium pur gene expression.

Journal of Bacteriology
U Houlberg, K F Jensen

Abstract

Data are presented which indicate that the repression of pur gene expression seen after the addition of preformed purines to cultures of Salmonella typhimurium is the consequence of the presence or the formation of the purine bases, hypoxanthine and guanine. This conclusion is based on the following observations. First, it was impossible to find a correlation between the size of any individual purine nucleotide pool and the level of the first four enzymes in the de novo biosynthetic pathway. Second, adenine plus guanosine served as a perfect source of purine nucleotides, but their presence caused no repression of pur gene expression if the cells lacked purine nucleoside phosphorylase activity. This enzyme is needed to convert adenine and guanosine to hypoxanthine and guanine, but not for their conversion to nucleotides. Third, addition of guanine to a strain lacking guanine phosphoribosyltransferase (gpt) resulted in a repression of the level of the purine de novo biosynthetic enzymes, a reduction of the growth rate, and a fall in the pools of ATP and GTP. Addition of hypoxanthine to a strain lacking hypoxanthine phosphoribosyltransferase (hpt) had a similar, although weaker, effect. If the cells lacked both hypoxanthine and gu...Continue Reading

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Citations

Sep 15, 2000·Journal of Bacteriology·J L ZillesD M Downs
Mar 22, 2014·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Tomohiro ShimadaAkira Ishihama
Dec 30, 2016·Microbiology and Molecular Biology Reviews : MMBR·Bjarne Hove-JensenMartin Willemoës
May 11, 2005·Molecular Microbiology·Lakshmi RajagopalC E Rubens
May 17, 2011·Nucleic Acids Research·Byung-Kwan ChoBernhard Ø Palsson
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Jun 25, 2008·Annual Review of Biophysics·Rebecca K Montange, Robert T Batey
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Dec 11, 1997·Journal of Bacteriology·B S ShinH Zalkin
Sep 1, 2008·EcoSal Plus·Kaj Frank JensenMartin WillemoËs

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